Neonatal septicaemia: Its bacteriological profile and antibiogram
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2016, Vol 2, Issue 4
Abstract
Background and Objectives: Neonatal septicaemia is a life threatening clinical emergency that demands urgent diagnosis and treatment. A wide variety of bacteria can cause neonatal septicaemia. As neonatal septicaemia is a life-threatening clinical emergency, the knowledge of epidemiological and antimicrobial susceptibility pattern of common pathogens in a given area helps to inform the choice of antibiotics, therefore a regular surveillance of important bacterial isolates and their susceptibility pattern is mandatory. So the present study was undertaken to identify the common bacterial pathogens associated with neonatal sepsis and their antibiotic susceptibility pattern. Material and Methods: The study was carried out in the Department of Microbiology, a tertiary care hospital Jalgaon, Maharashtra during the period from December 2015 to May 2016. A total 200 clinical suspected cases of neonatal septicemia were included in the study. Blood samples were collected with all aseptic precautions and inoculated on brain heart infusion broth (BHIB).Subculture were made on blood agar and Mac-conkey agar plates. Organisms were identified as per standard recommended procedures and antibiotic susceptibility testing was carried out on isolates as per Clinical Laboratory Standard Institute (CLSI) guidelines. Result: 60 different types of microrganisms were isolated. The incidence of neonatal septicemia was 30%.The prevalence of gram negative bacilli were 60.00% and gram positive bacilli were 40.00%.The commonest pathogen isolated was Klebsiella pneumonia 50.00%, followed by Staphylococcus aureus 30.00%. Klebsiella pneumonia was resistant to amikacin (86.66%) &100% resistant to ampicillin, and was 100% sensitive to pipercillin tazobactam and co-trimaxozole. The most effective antibiotics against the isolated Staphylococcus aureus were Vancomycin (100%), Linzeloid(100%), Ciprofloxacin (66%) ,Co-trimoxazole (66%) and Clindamycin (55%). Conclusion: The present study reveals microbiological profile and antibiogram of neonatal septicemia. Considering the burden of mortality resulting from septicemia, better diagnostic facilities should be employed for early detection of septicemia and rational use of narrow spectrum antibiotics is recommended.
Authors and Affiliations
Santosh Kotgire, Sunil Hatkar
Keywords
Related Articles
- EP ID EP243124
- DOI -
- Views 74
- Downloads 0
Evaluation of Malaria Surveillance System in Hooghly district of West Bengal - India
Background: Malaria is endemic in 109 countries worldwide leading to approximately 1 million deaths. In 2009, West Bengal reported 89443 malaria cases including 24453 falciparum malaria. Hooghly district had ABER in the...
Evaluation of purity, viability, stability and biochemical characteristics of clinical fungal isolates preserved by three different methods
Introduction In era of organ transplants and immunocompromised patients systemic mycosis has emerged as a major cause of morbidity and mortality among these patients Research on these clinical fungal isolates is the key...
Phenotypic detection of ESBL among E.coli and Klebiella pneumoniae by modified CLSI guidelines
Introduction Escherichia coli and Klebsiella pneumoniae cause a wide range of infections Multidrugresistance strains carrying resistance genes have become a growing problem worldwide The ESBLs have emerged distinctly esp...
Antibiotic profile of enterobacter isolates from various clinical samples – A study from a tertiary care hospital
Introduction Enterobacter species especially Enterobacter cloacae are important nosocomial pathogens They cause infections of the lower respiratory tract skin soft tissue urinary tract and occasionally sepsis Enterobacte...
Resistant Genes blaCTX-M, blaTEM and blaSHV encoding ESBL in surgical site infection causing Escherichia coli and Klebsiella pneumoniae- A report from a tertiary care hospital
Introduction Surgical site Infections SSI rank third among hospital acquired infections worldwide According to the WHO report of 2016 the global burden of SSI ranges from 25 to 415 ESBL producing Ecoli and Klebsiella pne...