Neuropharmacological study of Humboldtia vahliana Wight

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Neuropharmacological study of Humboldtia vahliana Wight

Journal Title: Scholars Academic Journal of Pharmacy - Year 2018, Vol 7, Issue 4

Abstract

Abstract: The present study was aimed to investigate ethanol and aqueous extracts of barks of Humboldtia vahliana Wight (Caesalpiniaceae) for various neuropharmacological activities, such as sedative and hypnotics, anticonvulsants, antianxiety and antidepressants with estimation of brain neurotransmitter level by using animal models. Extracts were obtained by continuous hot extraction using solvents with different polarities. Two doses (200, 400mg/kg) of ethanol and aqueous extracts of Humboldtia vahliana Wight was orally administered for one time to evaluate sleep latency and sleep time in Phenobarbitone Sodium induced sleep in mice, maximum electric shock induced seizure for the assessment of anticonvulsant activity where time duration of various phases in epilepsy were noted. Open field test for antianxiety activity, in which number square crossed with respect to cut off time and in forced swim test, immobility time duration was monitored for antidepressant activity. The extract which had highly significant effect was subjected to HPTLC study. After administration of extracts, restored the altered parameters to normal when compared to positive control group. The ethanol and aqueous extracts showed no signs of mortality on mice in acute toxicity studies at 2g/kg. Administered extracts shortened the sleep latency and prolonged the sleep time in phenobarbitone sodium induced sleep, in EEHV (400mg/kg)(p.o) the sleep latency was 14±1.42 and the duration of sleep was 163.20±4.02. Similarly reduced the time of various phases of seizure in MES induced seizures especially extensor phase, the duration of time(sec) for flexion , extensor, clonus, stupor and recovery was 1.50±0.28, 10.0±0.40, 13.25±0.48, 31.5±0.64 and 96.0±0.71 for EEHV(400mg/kg)(p.o). The doses of extracts showed significant anxiolytic activity by reduction in locomotion in open field test as in EEHV(400mg/kg)(p.o) the duration if time(min) from 0, 30, 60, 90 and 120 was reduced as 45.0±1.29 , 34.01±1.47, 25.0±1.29 ,16.25±1.10, 3.50±0.65 and the dose of antidepressant activity increased the immobility time(min) of mice in forced swim test as 223.25±1.38 for EEHV(400mg/kg)(p.o). It also restored the brain neurotransmitter such as adrenaline and dopamine to more or less to normal level. HPTLC fingerprint revealed the presence of six peaks at 254 and 366nm respectively. The results revealed that ethanol extract of H. vahliana possesses significant effect on neurological disorder than aqueous extract. The ethanol extract at dose 400 mg/kg body weight was found to be more effective than lower dose. This effect is due to the presence of phytoconstituents in these plants like, flavonoids, saponins and phenolic compounds

Authors and Affiliations

Raju Asirvatham, Sinchu Yesudanam

Keywords

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EP ID EP480158
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