Neuroprotective effect of N-acetylcysteine in neurons exposed to arachidonic acid during simulated ischemia in vitro.
Journal Title: Pharmacological Reports - Year 2009, Vol 61, Issue 4
Abstract
The aim of the study was to assess neuroprotective effects of N-acetylcysteine (NAC; 100-200 muM) on cultured cortical neurons exposed to arachidonic acid (AA, 10 muM) during ischemia (oxygen-glucose deprivation). Ischemic conditions decreased neuron viability to 41-47% of normoxic controls; co-exposure with arachidonic acid further attenuated neuron viability to 36.73% after 24 h. Separate exposure to arachidonic acid in normoxia or to ischemic conditions only, increased the number of apoptotic nuclei to 33.56% or 36.78%, respectively. Combined exposure to arachidonic acid and ischemia increased apoptosis frequency to 62.20%. NAC (200 muM) decreased the number of apoptotic nuclei in normoxia in control and arachidonic acid exposed cells. NAC also decreased apoptosis frequency in ischemia to 14%. In neurons exposed to arachidonic acid and ischemic conditions, 100 and 200 muM NAC reduced apoptosis to 24.99% and 19.48%, respectively. NAC provided protection to neurons from toxicity due to arachidonic acid, ischemia and exposure to arachidonic acid in ischemic conditions.
Authors and Affiliations
Natalia Pawlas, Andrzej Małecki
Keywords
Related Articles
- EP ID EP96885
- DOI -
- Views 101
- Downloads 0
N-phenylmaleimide derivatives as mimetic agents of the pro-inflammatory process: myeloperoxidase activation.
Myeloperoxidase (MPO) is an important enzyme that catalyzes the reaction between hydrogen peroxide and chloride to generate hypochlorous acid, which oxidizes a range of biomolecules and has been associated with inflammat...
Inhibition of NAD(P)H oxidase attenuates aggregation of platelets from high-risk cardiac patients with aspirin resistance.
Up to one-third of serious vascular events in high-risk patients is attributable to a failure of aspirin (ASA) to suppress platelet aggregation. We hypothesized that inhibition of NAD(P)H oxidase may inhibit aggregation...
Negative influence of L-dopa on subjectively assessed sleep but not on nocturnal polysomnography in Parkinson's disease.
Background: Sleep disorders are highly prevalent among patients with Parkinson's disease (PD). Chronic medication with L-dopa may be one of the factors that contributes to poor sleep quality. The aim of this study was to...
Abnormalities of the resistance vasculature in hypertension: correction by vasodilator therapy.
The structure of the resistance vessels in patients with essential hypertension is altered, with reduction of the lumen diameter and increase in wall-to-lumen ratio without change inwall mass, known as eutrophic remodell...
Effect of rutin on total antioxidant status of rats exposed to cigarette smoke.
Exposure to tobacco smoke impairs the antioxidant defense mechanisms. In female Wistar rats fed on regular rodent chow and supplemented with a flavonoids rutin, Trolox Equivalent Antioxidant Capacity (TEAC) was measured...