NEW IDENTIFICATION OF PHAGES IN WATER AND IN FISHES FROM LAGOON EBRIÉ AND THEIR APPLICATION FOR MULTIDRUG STRAINS IN ABIDJAN, WEST AFRICA
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 9
Abstract
Phages are widely apply to screen, to detect and to eliminate bacterial in different applications. In Africa, the emergence of antibiotic resistance strains and the fecal contamination in the aquatic environment require to explore the phage therapy. In Abidjan, the Lagoon Ebrié is an important aquatic ecosystem and the increase of the microbial contamination represents a risk for the population and the fisheries activity. The aim of this study is to identify novel phages in water and in fish samples from the Lagoon and to test their virulence against multidrug resistant strains. Water samples from different lagoon sites were collected and analyzed in culture and phages test assay. Fresh fishes were collected from Lagoon’s fish market in Abobodoumé/Locodjoro site. Water samples (PWLO6, PWR7, PWAD8) and intestinal solution were passed through 0.45 μm syringe filters and incubated in bacteria growth.. The enrichment of bacteria and phages were done in LB media at 37°C. We have isolated 5 phages from different types of fishes and 3 phages from Lagoon sites. The phages of fishes PFSO1, PFMU2, PFCAR4 and PFCAP5 have positive virulence for all tested antibiotic multidrug resistant strains Pseudomonas aeruginosa, Salmonella sp, Enterobacter aerogenes, Klebsiella pneumoniae and E. coli. The phage PFMA3 has shown not effect against Klebsiella pneumoniae and Enterobacter aerogenes. 3 phages from water have identical virulence effect in all tested bacteria. This study report the first isolation of 8 novel phages in Lagoon Ebrié with virulent effect on multidrug resistant’s strains and can be apply as a new approach for eliminating of pathogens.
Authors and Affiliations
Prof. Kakou Ngazoa E. Solange
Keywords
Related Articles
- EP ID EP618035
- DOI -
- Views 164
- Downloads 0
PRIMARY NON-HODGKIN'S LYMPHOMA OF UTERINE CERVIX
Primary non-Hodgkin's lymphoma of the uterine cervix is rare. We report a 56-year-old woman who presented with post-coital bleeding. Pelvic examination revealed a uterine cervical mass, which confirmed to be large B cell...
ASSESSMENT OF MOTHER KNOWLEDGE TOWARDS INTESTINAL PARASITE AMONG CHILDREN AT SCHOOL AGE
Introduction: - Deterioration of the health situation and lack of health services has led to a rise the occurrence of intestinal parasites between school students and particularly among nursery school and kindergarten. O...
EFFECTS OF HIBISCUS SABDARIFFA CALYX EXTRACT ON MALE REPRODUCTIVE ORGAN WEIGHT AND TESTOSTERONE IN NORMAL WISTAR RAT
The continuous use of plants in folklore for reproductive health and the traditional belief of Hibiscus sabdariffa as aphrodisiac have prompted this research work. Hence, the effect of oral administration of aqueous extr...
DISCOMYCETES OF THE ASCOMYCETES GROUP
Ascomycetes are the members which have the coenocytic mycelium and the septate branched mycelium and the dikaryotic stages in the life cycles. The structure of the fruiting body of the plectenomycetes types, the fruiting...
X GENE VARIABILITY AND GENOTYPING OF HEPATITIS B VIRUS (HBV) IN SUDANESE PATIENTS WITH LIVER DISEASES, KHARTOUM, SUDAN
Background: HBx gene mutations may have a role in the progression of liver disease from chronic infection to liver cirrhosis and/or hepatocellular carcinoma. These mutations affect the biological functions of HBx protein...