New Insight in Neuron Regeneration: Induction of Glia Cell to Neuron Cell
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 2
Abstract
ABSTRACT Induction neuron from a veriety of cell resource were remaining challeng in regenerative medicine, so finding the convenient method to reprogram different cells to neuron could be helpful. In this study, we analysis the transcriptome of glia and neuron cells to determine the gene expression in neuron that different when compare to glia cells. Then based on this transcriptom data seek the transcription factor and miRNA. Data extract from transcriptome database of mouse cells comprise cerebral cortex that generated by RNAseq technique. By comparison neuron against glia cells (astrocyte, oligodenderocyte and microglia) determined different gene expression in neuron. By using genetrail2 database determined transcription factor and miRNA associated with neuron gene expression.Result determined the 500 genes with different expression in neuron in comparison with glia cells. 2 significant TF families, DLX and MSX, 3 TF, Sp1, Ctcf and Pax1, 85 miRNA release from analysis this 500 gene. Analysis the gene target of all identified miRNA represent the important biological process related to neurogenesis neurodevelopment, in addition to most important proteins like Dnm1, Gad1 and Grin1 were obtained by functional and structural of network analysis. Dnm1 and Grin1 regulated by Sp1. In sum up, since one of the methods to reprogramming resident glia cells to induce neuron is applying TF and miRNAs, TF like Sp1 lonely or in combination with other factors can be experimentally approved.
Authors and Affiliations
Morteza Aliashrafi1, Hakimeh Zali2,* and Ameneh Jafari3
Keywords
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