New polymorphisms and expression of the porcine ghrelin (GHRL) gene in different pig breeds
Journal Title: Journal of Animal and Feed Sciences - Year 2011, Vol 20, Issue 2
Abstract
Growth and development traits are economically important in animal production, especially in pig breeding. Therefore, the porcine GHRL gene is considered as a candidate gene responsible for growth rate and body weight. The aim of our study was to identify new polymorphisms in the GHRL gene in pig. Ten novel single nucleotide polymorphisms (SNP’s) were detected: four substitutions in exons, four in introns and two mutations in promoter region. We evaluated the GHRL mRNA abundance in porcine stomachs (fundus ventriculis) and ghrelin protein concentration in plasma in three breeds: Polish Landrace, Polish Large White and Pietrain. The results showed that transcript abundance of GHRL gene was significantly higher in Polish Landrace than in other two breeds (P<0.05). The mutation c.-93A>G located in the promoter region affected expression of the GHRL gene. The AA genotype animals showed a significantly (P<0.05) higher expression level when compared to AG genotype animals.
Authors and Affiliations
K. Ropka-Molik, M. Oczkowicz, J. Romanek
Keywords
Related Articles
- EP ID EP74840
- DOI -
- Views 92
- Downloads 0
The effects of dietary cholecalciferol and 1α-hydroxycholecalciferol levels in a calcium- and phosphorus-deficient diet on growth performance and tibia quality of growing broilers
This study aims to evaluate the effects of cholecalciferol (vitamin D3, 0, 5, 10, 25, 50, 100, 250, 500, and 1000 mg · kg–1) and 1α-hydroxycholecalciferol (1a-OH D3, 0, 2.5, and 5 mg · kg–1) on growth performance and ti...
Evaluation of dry matter intake, average daily gain and faecal nitrogen excretion predicted by the Cornell Net Carbohydrate and Protein System with different beef cattle breeds fed in China
This study was conducted to evaluate the predictions of dry matter intake (DMI), average daily gain (ADG), and faecal nitrogen (N) excretion by the Cornell Net Carbohydrate and Protein System Version 6.1.26 (CNCPSv6) in...
Effect of enzyme addition, germination, and fermentation on the nutritive value of barley for growing Japanese quails
Two experiments were conducted to study the effect of fermentation and germination on the nutritive value of barley for Japanese quail. In the first experiment, 72 male 13-week-old Japanese quails were used to determine...
Does central IL-1β affect GnRH secretion in the hypothalamus of anoestrous ewes via different regulatory pathways?
The study was designed to identify the central pathways through which central interleukin-1β (IL-1β) affects gonadotropin-releasing hormone (GnRH) release in anoestrous ewes. Our results show that intracerebroventricular...
Entropy analysis of performance test results of warmblood stallions
The objective of the present study was to evaluate relationships among recorded traits in a 100-day stationary performance test in warmblood stallions in Poland. The possibility of reducing the number of recorded traits...