NEW RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DESVENLAFAXINE AND CLONAZEPAM IN TABLETS
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 2
Abstract
A simple, specific and precise high performance liquid chromatographic method was developed and validated for simultaneous determination of Desvenlafaxine and Clonazepam in tablets. The chromatographic separation was performed using Hypersil ODS C18 Column (4.6 × 250 mm, 5μm particle size). The mobile phase consisted of a combination of acetonitrile and 0.05 M ortho phosphate buffer (pH 4.0) in the ratio of 60:40 v/v at a flow rate of 1.2 ml/min with the detection wavelength at 225 nm. Both the drugs showed good linearity in the concentration range of 60-140μg/ml and 0.6-1.4μg/ml respectively. The correlation coefficients were obtained as 0.996 and 0.997 respectively for Desvenlafaxine and Clonazepam. The retention times for Desvenlafaxine and Clonazepam were found to be 2.687 and 3.817 min respectively. The developed analytical method was validated for linearity, precision, accuracy, ruggedness, robustness, specificity and system suitability according to ICH guidelines. The developed method can successfully be employed for routine quality control of Desvenlafaxine and Clonazepam in combined tablet dosage forms.
Authors and Affiliations
Usha Rani N, Sahithi G, Divya K
Keywords
Related Articles
- EP ID EP625066
- DOI -
- Views 73
- Downloads 0
GAS CHROMATOGRAPHY METHOD OF CLEANING VALIDATION PROCESS FOR 2-PROPANOL RESIDUE DETERMINATION IN PHARMACEUTICAL MANUFACTURING EQUIPMENT
Cleaning validation is an integral operation of good manufacturing practice in pharmaceutical industry. The aim of this study was to validate simple analytical method for detection of 2-propanol residue in equipment, whi...
STANDARDIZATION OF FENNEL (FOENICULUM VULGARE), ITS OLEORESIN AND MARKETED AYURVEDIC DOSAGE FORMS
Fennel, Foeniculum vulgare, one of the most common use in India kitchen as a spice and as well as in traditional medicine for its estrogenic, lactagouge, diuretic, antioxidant, immune booster and its usefulness in dyspep...
DEVELOPMENT AND CHARACTERIZATION OF HYDROXYL CHLOROQUINE SULPHATE (HCQ) NANOPARTICLES
The objective of present study was to formulate and characterize the nanoparticles of hydroxyl chloroquine sulphate (HCQ). Nanoparticles were prepared by the w/o/w emulsion - solvent evaporation method using polymer Eudr...
PHARMACOGNOSTICAL EVALUATION OF ROOTS OF SIMMONDSIA CHINENSIS SCHNEIDER
Simmondsia chinensis Schneider commonly known as jojoba is a semi-arid evergreen shrub. Jojoba is unique among plants in the fact that its products (seeds) contain about 50 percent by weight oil. Jojoba liquid wax genera...
NEUROINFLAMMATION IN ALZHEIMER’S DISEASE AND INVOLVEMENT OF INTERLEUKIN-1: A MECHANISTIC VIEW
Ageing is a universal biological fact and a natural process. It begins from the day we are born, or perhaps even before. Of the world’s 580 million elderly, 77 million (22% of total) live in India. The increasing life ex...