Nitric Oxide Levels And Total Antioxidant Capacity In The Seminal Plasma Of Infertile Smoking Men
Journal Title: Cell Journal(Yakhteh) - Year 2015, Vol 17, Issue 1
Abstract
Objective: Cigarette is a rich source of oxidants and reactive nitrogen species. Nitric oxide (NO) in high concentration has deleterious effects on human sperm function. Antioxidant defense system in seminal plasma protects spermatozoa from the attack of reactive oxygen metabolites. The purpose of this study was to determine the correlation between cigarette smoking with the NO levels and the total antioxidant capacity (TAC) of the seminal plasma in infertile smoker men and to compare severity of oxidative stress (OS) in them with fertile and infertile non-smoking men. Materials And Methods: In this cross sectional study, a total of 95 male participants attended the Infertility Clinic of the Fatehmeh-zahra Hospital in Babol, Mazandaran Province, Iran, between 2010 and 2011. They were divided into three groups: I. fertile non-smokers (F.ns; n=32), II. infertile non-smokers (IF.ns; n=30) and III. infertile smokers (IF.s; n=33) according to semen analysis World Health Organization guidelines (WHO, 2001) and smoking data. TAC concentration and NO levels of seminal plasma were measured using the ferric reducing ability of plasma (FRAP) method and the Griess reagent, respectively. Results: Standard sperm parameters were significantly higher in the fertile group than those in the infertile groups, but these differences between the IF.ns and IF.s were not statistically significant. The mean TAC in the seminal plasma was higher in the F.ns>IF.ns>IF.s, respectively, but these differences were not statistically significant among three groups (p= 0.096). In contrast, the mean NO level in the seminal plasma was lower in the F.ns<IF.ns< IF.s, respectively. These differences were statistically significant among the three groups (p= 0.018). Conclusion: It argued that the increased NO levels associated with smoking might exceed the capacity of antioxidant defense system, leading to increased oxidative damage of seminal plasma and decreased fertility in men.
Authors and Affiliations
Yousefreza Yousefniapasha, Gholamali Jorsaraei, Maryam Gholinezhadchari, Soleiman Mahjoub, Mahmoud Hajiahmadi, Mehrdad Farsi
Keywords
Related Articles
- EP ID EP593468
- DOI 10.22074/cellj.2015.519
- Views 150
- Downloads 0
Suppressive Effects of Resveratrol Treatment on The Intrinsic Evoked Excitability of CA1 Pyramidal Neurons
Objective: Resveratrol, a phytoalexin, has a wide range of desirable biological actions. Despite a growing body of evidence indicating that resveratrol induces changes in neuronal function, little effort, if any, has bee...
Comparison of Different Electroporation Parameters on Transfection Efficiency of Sheep Testicular Cells
Objective: Electroporation can be a highly efficient method for introducing the foreign genetic materials into the targeted cells for transient and/or permanent genetic modification. Considering the application of this t...
Indoleamine 2,3-Dioxygenase Is Dispensable for The Immunomodulatory Function of Stem Cells from Human Exfoliated Deciduous Teeth
Objective: In this study, we sought to better understand the immunoregulatory function of stem cells derived from human exfoliated deciduous teeth (SHED). We studied the role of the interferon gamma (IFN-γ)-indoleamine 2...
A Simple Technique for Three-Dimensional Imaging and Segmentation of Brain Vasculature Using Fast Free-of-Acrylamide Clearing Tissue in Murine
Objective Fast Free-of-Acrylamide Clearing Tissue (FACT) is a recently developed protocol for the whole tissue three-dimensional (3D) imaging. The FACT protocol clears lipids using sodium dodecyl sulfate (SDS) to increas...
The Effect of Mir-451 Upregulation on Erythroid Lineage Differentiation of Murine Embryonic Stem Cells
Objective MicroRNAs (miRNAs) are small endogenous non-coding regulatory RNAs that control mRNAs post-transcriptionally. Several mouse stem cells miRNAs are cloned differentially regulated in different hematopoietic linea...