Nitric oxide modulates the amphetamine effect on [(3)H]glucose uptake in the brain of rats prenatally exposed to lead.
Journal Title: Pharmacological Reports - Year 2007, Vol 59, Issue 5
Abstract
Glucose is the main source of energy for the central nervous system (CNS). In this study, we examined the effects of the psychostimulant amphetamine (AMPH) and the neuronal mediator nitric oxide (NO) on [(3)H]glucose uptake in the brain of adult rats that had been prenatally exposed to lead. Lead [Pb(CH(3)COO)(2) . 3H(2)O; 250 ppm] was added to the drinking water of pregnantWistar rats for the duration of pregnancy. On the day of parturition, lead was discontinued as an additive in the drinking water. Offspring remained ith dams for 21 days. The control group consisted of rats that consumed water without lead. In adulthood, male offspring from both groups (lead-exposed and control) were pretreated with 7-nitroindazole (nNOS blocking agent) (10.0 mg/kg ip) or saline (1.0 ml/kg ip), 30 min before AMPH (1.0 mg/kg ip). After another 30 min, and 15 min before termination, all rats were injected with 6-[(3)H]-D-glucose (500 muCi/kg ip). Brain specimens were taken (striatum, frontal cortex, hippocampus, and thalamus with hypothalamus, and pons with medulla oblongata) for determination of radioactivity in a liquid scintillation counter.We found that lead did not alter [(3)H]glucose uptake in brain regions studied (with exception of frontal cortex) but that AMPH increased [(3)H]glucose uptake in the striatum, frontal cortex and hippocampus, and that the AMPH effect was lessened in the hippocampus of lead-exposed rats. Moreover, the AMPH effect on [(3)H]glucose uptake in the frontal cortex, hippocampus, thalamus with hypothalamus and pons of control rats was potentiated by 7-NI pretreatment. Similar effect was observed in lead-intoxicated rats (striatum, frontal cortex and hippocampus). These results indicate that NO modulates AMPH-induced [(3)H]glucose uptake in the brain of rats prenatally exposed to lead.
Authors and Affiliations
Przemysław Nowak, Marek Swoboda, Ryszard Szkilnik, Jadwiga Jośko, Łukasz Noras, Adam Kwieciński, Katarzyna Sawczuk, Ewa Herba, Dorota Pojda-Wilczek, Richard Kostrzewa
Keywords
Related Articles
- EP ID EP154757
- DOI -
- Views 94
- Downloads 0
Influence of vitamin C on markers of oxidative stress in the earliest period of ischemic stroke.
Experimental studies have demonstrated that oxidative stress plays an essential role in the pathophysiology of ischemic stroke. The objective of the present study was to assess some serum markers of oxidative stress in p...
Regulation of LDL receptor expression by the effect of curcumin on sterol regulatory element pathway.
To investigate the molecular mechanisms of the effect of curcumin on up-regulation of LDL receptor expression, a sterol regulatory report system was established in Xenopus laevis oocytes by microinjection of a plasmid pL...
Ebselen attenuates oxidative stress in ischemic astrocytes depleted of glutathione. Comparison with glutathione precursors.
In this study, we investigated the protective effect of ebselen, a seleno-organic compound with antioxidant activity, towards astrocyte degeneration caused by exposure to simulated in vitro ischemic conditions and simult...
Monocyte suppressing action of fenofibrate.
Since atherosclerosis has been proven to be an inflammatory disease, itis obvious that the proper treatment for dyslipidemia should not only correct lipid parameters but alsoinhibit inflammation. Monocytes and monocyte-d...
Biological actions of lipoic acid associated with sulfane sulfur metabolism.
This work aimed to investigate the effect of lipoic acid (LA) on sulfane sulfur (S(*)) level and rhodanese activity in rat tissues. In vitro studies conduced so far have indicated that dihydrolipoic acid serves as an S(*...