Nitric oxide signaling in the ischemic postconditioning of human heart muscle (RCD code: III)
Journal Title: Journal of Rare Cardiovascular Diseases - Year 2016, Vol 2, Issue 6
Abstract
Background: Ischemic heart conditioning is well documented to trigger the intrinsic protective mechanisms of resistance against ischemia/reperfusion (I/R) injury. Previous studies on animal model have suggested that the nitric oxide (NO) mediates the beneficial effect of ischemic postconditioning (POC). We tested the hypothesis that POC provide cardioprotection in the NO-dependent mechanism in the human myocardium. Methods: Human atrial trabeculae were subjected to simulated I/R injury. To achieve POC triple brief hypoxia periods were followed by the lethal hypoxia. Non-selective inhibitor of NO synthase: NG-monomethyl-L-arginine (L-NMMA) was used at the time of re-oxygenation in the POC protocol. Contractility of the myocardium was assessed as the maximal force of a contraction (Amax), the rate of rise of the force of a contraction (Slope L) and cardiac muscle relaxation – as the rate of decay of the force of a contraction (Slope T). Results: Co-application of L-NMMA with POC resulted in the decrease of Amax, Slope L and Slope T during re-oxygenation period as compared to POC only. Conclusions: At re-oxygenation period, the blockade of NO synthesis has deleterious effect on systolic and diastolic function of human myocardium, as well as attenuates the beneficial effect of ischemic.
Authors and Affiliations
Marcin Kunecki, Wojciech Płazak, Piotr Podolec, Tomasz Roleder, Jolanta Biernat, Marek Deja, Krzysztof Gołba
Keywords
Related Articles
- EP ID EP248094
- DOI 10.20418/jrcd.vol2no6.228
- Views 101
- Downloads 0
Senning operation as a palliative therapy for a girl with complex heart defect and Eisenmenger syndrome (RCD code: II‑1A.4d)
Development of irreversible pulmonary hypertension in D-transposition of the great arteries (D-TGA) with ventricular septal defect (VSD) is a well-known phenomenon. Coexistence of left ventricular outflow tract obstructi...
Journal of Rare Cardiovascular Diseases: Another exciting quarter in the ‘usual’ life of the Journal
Dear Friends and Colleagues,Our Dear Readers and Supporters of the Journal,It is an extreme pleasure to deliver at your hand the 7th issue of the Journal of Rare Cardiovascular Diseases. Our two‑year anniversary has not...
Severe course of dilated cardiomyopathy associated with Duchenne muscular dystrophy (RCD code: III‑1A.3a)
Inherited neuromuscular disorders, such as dystrophin-related disorders are frequently associated with cardiac involvement, mainly with cardiomyopathies. Dystrophin-related disorders are caused by the mutation in the dys...
Long QT syndrome type 2 – can alarm clock really kill? (RCD code: V-1A.2)
This paper’s aim was to critically analyze existing research supporting statement found in many review articles that auditory stimuli may specifically and exclusively trigger cardiac events in patients with type 2 of con...
Successful reversal of advanced heart failure due to peripartum cardiomyopathy with aggressive pharmacotherapy and a continuous‑flow left ventricular assist device (RCD code: III-1B.8c)
Peripartum cardiomyopathy (PPCM) is a rare but potentially devastating complication of pregnancy. PPCM is not well understood and relatively little is known about its epidemiology and pathogenesis. However, it appears to...