NO-SYNTHASE ACTIVITY OF PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH ACNE VULGARIS
Journal Title: Мікробіологія і біотехнологія - Year 2018, Vol 0, Issue 4
Abstract
The aim is to study changes in the activity of distinct NO-synthase isoforms of blood lymphocytes in persons with acne vulgaris, for those individuals with disease accompanied by seeding of film-forming and planktonic staphylococcus forms. Enzyme activity was determined both before and after treatment. Methods. 44 patients with acne vulgaris were studied, for that persons the cultures of filmforming and planktonic forms of Staphylococcus aureus and Staphylococcus epidermidis were isolated from purulent pustules. Separation of lymphocytes from fresh peripheral heparinized blood of patients (before and after treatment) and individuals in the control group was done in the ficol triumbras (ρ=1.08 g/cm3 ). NO-synthase activity was expressed as citrulline production in picomoles per 1 milligram of protein per 1 minute. Results. Slightly higher rates of leukocyte іNOS were detected with the participation of biofilm staphylococci for S. aureus and S. epidermidis species compared to the planktonic forms of these staphylococcal species. For the acne caused both by S. aureus and S. epidermidis (planktonic and film-forming form) it was shown the significant increase ofendothelial NO-synthase activity (3.8–5.2 times) and inducible NO-synthas activity (52.5–55.1 times) ofperiferial blood leukocytes compared to control. The activity of the inducible isoform NO synthase significantly decreases relative to the control level after the course of acne treatment complicated by the proliferation of S. aureus (planktonic and film-forming form). Conclusions. The increase in the activity level of iNOS was observed with the participation of biofilm staphylococci of S. aureus and S. epidermidis species. In both forms of acne caused by S. aureus and S. epidermidis (planktonic and film-forming), the activity of NO-synthase isoforms increases with respect to control values. The activity of the inducible isoform NO-synthase can serve as a biomarker for monitoring treatment efficacy.
Authors and Affiliations
G. Lavryk, O. Korniychuk, Z. Fedorovych
Keywords
Related Articles
- EP ID EP491483
- DOI 10.18524/2307-4663.2018.4(44).149616
- Views 87
- Downloads 0
ELECTRIC CURRENT GENERATION BY DESULFUROMONAS ACETOXIDANS IMV B-7384 WHILE FERRIC CITRATE, FUCHSINE AND METHYLENE BLUE APPLICATION
Aim: to investigate the process of exoelectrogenesis performed by Desulfuromonas acetoxidans IMV B-7384 in a microbial fuel cell (MFC) under the presence of exogenous electron mediators (fuchsine, methylene blue) and Fe3...
EFFECT OF LACTOBACILLUS PLANTARUM ON THE AGENT OF DAMPING-OFF OF PINE SEEDLINGS
Aim. To study the effect of L. plantarum on micromycetes of Fusarium sp. strain isolated from Scots pine seedlings infected with damping-off disease. Methods. The antagonistic effect of lactobacilli was tested by well ag...
ANTAGONISTIC ACTIVITY OF ENDOSPOREFORMING BACTERIA OF DEEP WATER THE BLACK SEA SEDIMENTS
Aim. The purpose of the work was to study the antagonistic activity of strains of the facultatively anaerobic endosporeforming bacteria, isolated from deep-sea sediments of the Black Sea. Materials and methods. In this w...
ANALYSIS OF NEW POLYFLUOROTHIOACYLATED AMINO ACIDS DERIVATIVES BY IN SILICO AND IN VITRO METHODS
Aim. The purpose of this work was to analyze the potential biological activity and the target of action of esters of tetrafluoropropanethioacylated amino acids derivatives and to investigate the obtained results in vitro...
STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS
Purpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activit...