NO-SYNTHASE ACTIVITY OF PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH ACNE VULGARIS
Journal Title: Мікробіологія і біотехнологія - Year 2018, Vol 0, Issue 4
Abstract
The aim is to study changes in the activity of distinct NO-synthase isoforms of blood lymphocytes in persons with acne vulgaris, for those individuals with disease accompanied by seeding of film-forming and planktonic staphylococcus forms. Enzyme activity was determined both before and after treatment. Methods. 44 patients with acne vulgaris were studied, for that persons the cultures of filmforming and planktonic forms of Staphylococcus aureus and Staphylococcus epidermidis were isolated from purulent pustules. Separation of lymphocytes from fresh peripheral heparinized blood of patients (before and after treatment) and individuals in the control group was done in the ficol triumbras (ρ=1.08 g/cm3 ). NO-synthase activity was expressed as citrulline production in picomoles per 1 milligram of protein per 1 minute. Results. Slightly higher rates of leukocyte іNOS were detected with the participation of biofilm staphylococci for S. aureus and S. epidermidis species compared to the planktonic forms of these staphylococcal species. For the acne caused both by S. aureus and S. epidermidis (planktonic and film-forming form) it was shown the significant increase ofendothelial NO-synthase activity (3.8–5.2 times) and inducible NO-synthas activity (52.5–55.1 times) ofperiferial blood leukocytes compared to control. The activity of the inducible isoform NO synthase significantly decreases relative to the control level after the course of acne treatment complicated by the proliferation of S. aureus (planktonic and film-forming form). Conclusions. The increase in the activity level of iNOS was observed with the participation of biofilm staphylococci of S. aureus and S. epidermidis species. In both forms of acne caused by S. aureus and S. epidermidis (planktonic and film-forming), the activity of NO-synthase isoforms increases with respect to control values. The activity of the inducible isoform NO-synthase can serve as a biomarker for monitoring treatment efficacy.
Authors and Affiliations
G. Lavryk, O. Korniychuk, Z. Fedorovych
Keywords
Related Articles
- EP ID EP491483
- DOI 10.18524/2307-4663.2018.4(44).149616
- Views 93
- Downloads 0
CU (II) ACCUMULATION BY MARINE NEUTROPHIL SULFUR-OXIDIZING BACTERIA
Aim: to determine the ability of neutrophilic sulfur-oxidizing bacteria to extract Cu (II) ions from aqueous solutions. Methods. The object of the study was the ability of marine sulfur-oxidizing bacteria isolated from t...
EFFECT OF CAUSAL AGENT OF BASAL BACTERIOSIS ON SEED GERMINATION AND ROOT GROWTH OF DIFFERENT WHEAT VARIETIES
Aim. To investigate the effect of causal agent of basal bacteriosis Pseudomonas syringae pv. atrofaciens and its lipopolysacharide (LPS) on seed germination and growth of seedlings of various wheat varieties. Materials a...
STUDY OF CRYOINJURIES OF FREE AND IMMOBILIZED IN SODIUM ALGINATE GEL SACCHAROMYCES CEREVISIAE YEAST CELLS
The aim of this study was the revealing of the peculiarities of cryoinjuries of free and immobilized in sodium alginate gel Saccharomyces cerevisiae cells after cryopreservation. Methods. The research object was the Sacc...
CELLULOLYTIC ACTIVITY OF STRAIN OF THE FUNGUS ACREMONIUM SP. 502 PICKED UP FROM AFFECTED CUCUMBER PLANTS
The aim of the work was to study the cellulase activity of the strain Acremonium sp. 502 depending on medium pH, as a determinant of its penetration into the plant. Methods. The strain Acremonium sp. 502 was cultivated o...
FACULTATIVELY-ANAEROBIC ENDOSPOREFORMING BACTERIA OF DEEP WATER BOTTOM SEDIMENTS OF BLACK SEA
Aim of the work was to detect in deep-sea sediments of the hydrogen sulfide bathyal zone of the Black Sea the facultative-anaerobic spore-forming bacteria (FASB) capable of grow under aerobic conditions, and to determine...