Nonapoptotic cell death induced by Hypericum species on cancer cells
Journal Title: The European Research Journal - Year 2017, Vol 3, Issue 1
Abstract
Objective. There are approximately 400 Hypericum species that grow naturally in different geographic origins of the world. Those species have been used in different folk medicines and screened for their biological activity including cancer. Our country is an important place for Hypericum species which are known as “kantaron, binbirdelik otu, kan otu, kılıç otu, yaraotu, kuzukıran”. We therefore evaluated the possible cytotoxic/apoptotic activities, total phenolic content and antioxidant capacity of the crude methanol extracts Hypericum adenotrichum Spach. and Hypericum olympicum L. which are still used in Turkish folk medicine. Methods. The total phenolic content and antioxidant capacity were determined by Folin-Ciocalteu and ABTS methods. Anti-growth effects were screened in human hepatoma (Hep3B) and rat glioma (C6) cell lines by the MTT and ATP viability assays. The cell death mode (apoptosis/necrosis) was investigated by fluorescence imaging and the level of caspase-cleaved cytokeratin 18 (M30), active caspase-3 and cleaved PARP (poly (ADP-ribose) polymerase). Results. The results indicate that the crude methanol extracts of Hypericum olympicum L. and Hypericum adenotrichum have both anti-growth/cytotoxic activities on these cells in a dose dependent manner. Conclusion. These extracts clearly induced non-apoptotic cell death in human hepatoma (Hep3B) and rat glioma (C6) cell lines.
Authors and Affiliations
Ferda Ari, Nazlihan Aztopal, Merve Erkisa, Serap Celikler, Saliha Sahin, Engin Ulukaya
Keywords
Related Articles
- EP ID EP175757
- DOI 10.18621/eurj.292460
- Views 172
- Downloads 0
An interesting co-existence of celiac disease and idiopathic pulmonary hemosiderosis: Lane Hamilton syndrome
Idiopathic pulmonary hemosiderosis is characterized by hemoptysis resulting from recurring alveolar hemorrhage attacks, iron deficiency anemia, and parenchymal infiltrations as seen on chest radiographs. The clinical cou...
Toxoplasma gondii seropositivity in pregnancies with normal delivery and complicated with abortion
Objective: Toxoplasma infection during pregnancy may cause remarkable mortality and morbidityin fetus due to transplacental transmission. It has fetal consequences such as abortion, stillbirth and congenital malformation...
Increased TIMI frame count of coronary arteries in patients with myocardial bridging
Objectives: Myocardial bridging (MB) is associated with recurrent chest pain and cardiovascular events. Recently it has been proposed that MB has the features of vasospastic coronary artery characterized with reduced cor...
Surgical interventions for autogenous arteriovenous fistula aneurysms in hemodialysis patients
Objectives. The aim of this study was to emphasize the importance of surgical intervention perfotmed before development of arteriovenous fistula (AVF) aneurysm complications. Methods. The patients were categorized into...
The impact of cigarette dependence severity on disease outcomes and depression in ankylosing spondylitis
Introduction: Ankylosing spondylitis (AS) is a chronic systemic inflammatory disease. Smoking plays a role in AS pathogenesis and causes difficulties in its treatment. The aim of this study is to investigate the associat...