Novel Distribution of HCV genotypes and their Main Routs of Infection in the Punjab Pakistan
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2017, Vol 1, Issue 4
Abstract
Background: Hepatitis C is rapidly emerging as a major health problem in developing countries including Pakistan that leads to death and morbidities. HCV has a high genetic variation and is classified into six major genotypes and 67 subtypes. (Direct-Acting-Antiviral) anti-HCV drugs therapy response, resistance and recovery rates depend on HCV genotype.Methods: To study the epidemiological study of HCV genotypes in 2015-16 and their main routs of infection in the Punjab Pakistan. Observational study of the patients from 27 centers, 4823 samples was collected from HCV positive patients. History was noted on pre-design tested questioner which was approved by institutional Ethical committee. RNA was extracted using Favorgen Viral Nucleic Acid Extraction Kit. Amplification was performed using Amplisense Genotyping kit. We studied source of infection and therapeutic responses of varies genotypes on the basis of geographical distribution; we found a new incitement and provoke.Results: A total of 4823 serum samples were tested by type-specific genotyping assay. RNA was extracted using FoverGen Mini Kit. For HCV genotyping AmpliSens® HCV-genotype-FRT PCR kit variant FRT-g1-6. Detail history of each patient was taken on a predesigned questioner which was approved by Institutional Ethical Committee. Total 7800 individuals were analyzed by anti HCV ELISA out of which 5451 patient were found reactive. The positive samples were further conformed by PCR for HCV and their genotypes, out of which 4823 (88.47%) were found detected for HCV RNA. In the division of genotypes in Punjab varies from a maximum of 57.6% the genotype 3a, followed by 3b 14.76% on the other hand least common genotype was type-5 (0.14%). The major route of infection was surgery/dental procedures (52.02%), use of unsafe syringes (18.45%), blood transfusion (16.26%), razors or circumcision (5.90%), less than 3% due to needle stick, while 6.35% was unclear.Conclusion: HCV The most spread genotype in Pakistan was 3a with rate of 58% followed by genotype 3b and 1a, respectively. Dental surgery was the main source of infection
Authors and Affiliations
Sajjad Ullah, Fayyaz Ahmad, Ahmad Bilal Waqar, Arshad Jamal
Keywords
Related Articles
- EP ID EP569437
- DOI 10.26717/BJSTR.2017.01.000340
- Views 159
- Downloads 0
Breakthrough Treatment for Mantle Cell Lymphoma
MCL has a dismal prognosis (“the worst lymphoma to have”), with a median OS rate of 3 years only. Prognosis of limited stage disease is almost similar to that of stage lll MCL No curative therapy has been established so...
Treatment of Fingertip Gangrene in a Patient with Raynaud’s Phenomenon who Simultaneously Underwent Gastrectomy and Chemotherapy for Gastric Carcinoma: A Case Report
Raynaud’s phenomenon, characterized by recurrent vasospasm in the fingers and toes, is a common clinical disorder encountered by dermatologists. Hand-arm vibration syndrome is well known to cause secondary Raynaud’s phen...
Novel Distribution of HCV genotypes and their Main Routs of Infection in the Punjab Pakistan
Background: Hepatitis C is rapidly emerging as a major health problem in developing countries including Pakistan that leads to death and morbidities. HCV has a high genetic variation and is classified into six major geno...
Evodiamine Induces Extrinsic Apoptosis Through P38 Mapk Pathway and Inhibits Autophagy in Gallbladder Cancer
The gallbladder cancer is the most common malignant tumor of the biliary tract and the third most common gastrointestinal malignant tumor in the world for public health with extremely poor prognosis. The primary purpose...
Is Low Grade Infection a Contributing Factor of Disc Degeneration?
Intervertebral disc degeneration (IVD) has a multi factorial origin. Genetic and environmental factors are major determinants. However the pathomechanisms of IVD is inadequately understood. Low grade infection has also b...