NOVEL HPTLC-DENSITOMETRIC METHOD FOR THE ESTIMATION OF TERIFLUNOMIDE IN TABLET DOSAGE FORM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 6
Abstract
Objective: The current work is intended towards the development of a novel, simple and precise high-performance thin layer chromatographic (HPTLC) method coupled with a densitometer for the estimation of teriflunomide (TEF) present in the marketed formulation. Methods: The chromatographic development was performed on aluminum plates coated with silica gel 60 F254 using toluene: ethyl acetate: glacial acetic acid (7.5:2: 0.5 v/v/v) as the mobile phase. Densitometric scanning was achieved at the absorbance maxima, UV 284 nm. Results: Well separated band was observed with Rf value 0.46. The calibration curve plotted in the concentration range 100-700ng/band exhibited an excellent linear relationship with the r2 value of 0.9928. The method was found to comply with all the validation parameters as per the ICH guidelines. Conclusion: The method ensures minimal use of mobile phase with minimal run time compared to other reported analytical methods. This validated method can be used by quality control laboratories for the routine quantitative analysis of tablets consisting of Teriflunomide.
Authors and Affiliations
T. S. Vishwas, B. M. Gurupadayya, Rupshee Jain
Keywords
Related Articles
- EP ID EP578009
- DOI 10.22159/ijpps.2019v11i6.31742
- Views 86
- Downloads 0
TOTAL PHENOL, FLAVONOID AND ANTIOXIDANT PROPERTIES OF AURICULARIA AURICULA-JUDAE
Objective: From ancient times, Auricularia auricula has been used as food and also as herbal medicine in China. The hot water extract of Auricularia auricula fruiting bodies were evaluated for free radical scavenging pot...
COMMUNITY STRUCTURES OF ENDOPHYTIC ACTINOBACTERIA FROM MEDICINAL PLANT CENTELLA ASIATICA L. URBAN-BASED ON METAGENOMIC APPROACH
Objective: This study aimed to assess the community structure of actinobacteria in rhizosphere and endophyte of a medicinal plant, Centella asiatica, based on a metagenomic approach using Polymerase Chain Reaction-Denatu...
IN-VIVO ANTI-PYRETIC, ANTI-NOCICEPTIVE, NEUROPHARMACOLOGICAL ACTIVITIES AND ACUTE TOXICITY INVESTIGATIONS OF BLUMEA LACERA
Objective: The present study was done to evaluate in vivo anti-pyretic, neuropharmacological activity including open field and swimming test, gastrointestinal motility, anti-nociceptive and acute toxicity effect of diffe...
NOVEL HPLC-UV METHOD USING VOLATILE BUFFER FOR SIMULTANEOUS DETERMINATION OF AMLODIPINE BESYLATE AND ATORVASTATIN CALCIUM
Objective: The purpose of this work was to develop and validate a novel HPLC-UV method using triethylamine (TEA) as a volatile buffer for simultaneous determination of amlodipine besylate (AML) and atorvastatin calcium (...
STROKE OUTCOMES IN NON-DIABETIC, DENOVO DIABETIC AND DIABETIC INDIAN PATIENTS MEASURED BY MODIFIED RANKIN SCALE: AN OBSERVATIONAL STUDY
Objective: Hyperglycemia is a known risk factor which adversely impacts the outcomes in stroke patients compared to patients with normal blood glucose levels. Patients suffering from an acute stroke who are previously no...