Novel potential of pancreatic-like enzymes of microbial origin in exocrine pancreatic insufficiency – study on a pig model
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2015, Vol 9, Issue 1
Abstract
[b]Introduction.[/b] The standard porcine-derived pancreatic enzyme replacement therapy (PERT) is a lifesaving treatment for patients with diseases causing exocrine pancreatic insufficiency (EPI). An attempt to replace PERT with microbial enzymes were undertaken. The aim was to highlight whether the mode of application, mixed with food or applied directly to the stomach, of pancreatic-like enzymes of microbial origin (PLEM) can affect their activity along the gastrointestinal tract. [b]Materials and method[/b]. The activity of amylase, lipase and proteinase in the stomach, duodenum and ileum were tested in EPI pigs (n=6) after supplementation of PLEM, either orally – before and during feed consumption – or via the stomach – before and during feed consumption. Healthy pigs not treated with PLEM (n=3) served as controls. Activity of the enzymes measured in the chyme were obtained together with the digesta pH. Activity of the enzymatic residues in the stool samples was also checked. [b]Results[/b]. The highest pancreatic enzyme activities were found in the duodenum of the healthy pigs (amylase 162,68 kU/mL, lipase 507,34 kU/mL and protolitic (trypsin) activity 357,60 kU/mL). Nevertheless, the microbial enzymes remained also active along the entire length of the GIT – including stomach in EPI pigs, regardless of their route of administration. However, activity level was significantly lower. [b]Discussion.[/b] Results indicate that the activity pattern of PLEM in the small intestine mimics the activity of the natural endogenous pancreatic enzymes in healthy pigs. The most physiological features of PLEM were observed when enzymes were offered orally. The magnitude of PLEM activity in the stomach of EPI pigs was essential and significantly higher than that measured in healthy pigs, thus being somewhat not physiological, and for health reasons of the patients should be further explored. Interestingly, specific trypsin-like activity was measured in all parts of the GIT after PLEM application. However, proteolytic activity of the experimental proteaze in in vitro studies did not exhibit trypsin-like activity.
Authors and Affiliations
Katrin Szwiec, Kateryna Goncharova, Jose Luis Valverde-Piedra , Galyna Ushakova, Tetiana Kovalenko, Iryna Osadchenko, Marek Kardas, Paulina Świeboda, Paweł Podgurniak, Mateusz Winiarczyk, Elżbieta Grochulska-Niedworok, Liudmila Lozinska, Rafał Filip, Stefan G Pierzynowski
Keywords
Related Articles
- EP ID EP58830
- DOI -
- Views 123
- Downloads 0
Screening for anthrax occurrence in soil of flooded rural areas in Poland after rainfalls in spring 2010
[b]Introduction and objective[/b]. Anthrax spores remain viable and infectious in soil for decades. Flood water can percolate towards the surface the spores buried in soil. Moreover, the flood water might transport spor...
Evaluation of CD40 and CD80 receptors in the colonic mucosal membrane of children with inflammatory bowel disease
[b][/b][b]Introduction. [/b]The most prevalent inflammatory bowel diseases (IBD) include ulcerative colitis (UC) and Crohn’s disease (CD). Immune processes play a vital role in the etiopathogenesis of these conditions, i...
Designer Drug (DD) abuse in Poland; a review of the psychoactive and toxic properties of substances found from seizures of illegal drug products and the legal consequences thereof. Part II – Piperazines/Piperidines, Phenylethylamines, Tryptamines and miscellaneous ‘Others’
As the second and concluding part, this paper continues the summary review of the scientific evidence obtained from the literature and focuses on the remaining 4/6 groupings of DDs identified in illegal products found in...
Assessing physical activity and sedentary lifestyle behaviours for children and adolescents living in a district of Poland. What are the key determinants for improving health?
[b]Introduction.[/b] Adequate levels of physical activity throughout an individual’s life ensure an optimal state of health. Only 30% of adolescents and 10% of adults perform sufficient physical activity to facilitate pr...
Level and determinants of physical activity among school adolescents in Poland
The recognition of adolescents’ physical activity has become one of the serious challenges of the modern times, as a concern about the future health of societies. The major goal of the presented study was to discover the...