Observations of Enamel Microstructure in Incipient Lesions Remineralized by NaF Dentifrices
Journal Title: Dental Research and Management - Year 2017, Vol 2, Issue 1
Abstract
Synchrotron Radiation (SR) Wide-angle X-ray Diffraction (WAXD) and Small-angle X-ray Scattering (SAXS) techniques were used to assess microstructure of bovine enamel white-spot lesions (WSL) evaluated in a 10-day pH cycling model comprising three different dentifrice groups: (A) 0.21% NaF plus TCP (Clinpro Tooth Crème), (B) 1.1% NaF plus TCP (Clinpro 5000), or (C) 0% NaF (Tom’s of Maine) dentifrice. Each day consisted of four 2-minute treatments, one 4-hour acid challenge (pH=5.0), and immersion in artificial saliva (pH=7.0) between these events. These specimens were also examined with cross-sectional microhardness, digital light microscopy and FE-SEM (field emission scanning electron microscope), and demonstrated the remineralization model effected changes in subsurface microstructure. X-ray diffraction data from WAXD and SAXS were collected on enamel slab cross-sections extending from 0 μm to 150 μm, in 6 μm microbeam increments. A primary outcome of this observational study was that simultaneous WAXD and SAXS measurements were able to resolve significant differences (ANOVA, Student’s t-test, p<0.05) between the effects of the two fluoride-containing dentifrices on subsurface lesion microstructure. In particular, enamel lesions treated with 1.1% NaF dentifrice group manifested an abundance of nanometer-sized crystallites, while treatment with the 0.21% NaF dentifrice produced larger apatite-like crystals. While the presence of fluoride in both cases promoted regularity in crystal size and orientation, this was not observed for lesions treated without fluoride. Altogether, our observations demonstrate the pathological processes for remineralization are markedly influenced by the presence and concentration of fluoride, the microstructural characteristics of which can be distinguished using the simultaneous WAXD and SAXS technique.
Authors and Affiliations
Makoto Asaizumi
Keywords
Related Articles
- EP ID EP399264
- DOI -
- Views 230
- Downloads 0
Treatment of the Deep Overbite Malocclusion with Lingual Orthodontic Appliance during the Patient was Pregnant
While the mini-implant technique is common to use this technique to control vertical malocclusion, lingual orthodontic appliance is also a powerful method to deal with this malocclusion. And pregnancy is quite a challeng...
Comparison of Three Different Techniques to Allay Anxiety before Local Anesthesia Injection in Pediatric Population
Introduction: The fear of dentists is one of the major concerns why pediatric patients avoid dentists and thereby compromising their oral hygiene status. Injection of local anesthesia is considered to be the most dreaded...
Beginnings of University Dental Education in Croatia: A Short Historical Review
Improvement of biomedical sciences resulted with recognition of dental medicine as an independent profession, and establishment of dental schools in Europe and America. The founder of dental high educati...
The Prevention and Treatment of Neural Arterial Gingival Simplex
Neural Arterial Gingival Simplex is a common systemic disease linked to an invasive periodontal pathogen, Porphyromonas gingivalis as the key initiator. Instead of considering separate pathologic conditions as separate d...
No Antibiotics for Tooth Extractions in Diabetic Patients
Diabetes is a disease associated with increased surgical complications and deficient tissue repair. However, recent publications claim that non-compensated diabetic patients have not increased risk for pos...