Obtaining of microtubers of potato cv. ‘Andinita’ in Temporary Immersion Systems
Journal Title: Biotecnología Vegetal - Year 2011, Vol 11, Issue 1
Abstract
The Instituto Nacional de Investigaciones Agrícolas (INIA), Venezuela, is producing potato seed by biotechnology in its National Seed Plan. The seeds needed are greater than the installed capacity. The acquisition of temporary immersion system (ITS) enables developing research in the production of seed potatoes to ensure quality, efficiency and reduced production costs. The purchase of seeds will decrease and contribute to food security and sovereignty of the country. This work was aimed to obtain potato microtubers cv. ‘Andinita’ in SIT. In vitro plants propagated by organogenesis and SIT in 10 liters capacity were used. Explants (100) were inoculated per pot. After five weeks in multiplication a change of culture medium was carried out to induce tuberization. Three immersion frequencies were tested. Plant height was measured and the number of microtubers and fresh dough was quantified. Potato microtubers cv. ‘Andinita’ in SIT were obtained. The best results were achieved with immersions every four hours, averaging five to seven microtubers per plant (approximately 600 microtubers per culture vessel), with sizes between 4 and 16 mm, with an average of 3 g fresh weight, which ensured budding efficiency and allow direct field planting. This result constitutes the first report of the use of SIT for propagation of potatoes in Venezuela. This is a new possibility to use SIT in other varieties.
Authors and Affiliations
Janet Igarza Castro , Daniel Agramonte , Manuel de Feria , Juan Jaime , Miguel Pérez , Mario San Román
Keywords
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