Obtaining plant Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis
Journal Title: Plant varieties studying and protection - Year 2017, Vol 13, Issue 1
Abstract
Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.
Authors and Affiliations
Svitlana Gontarenko, Snizhana Lashuk
Productivity and adaptability of Myronivka spring barley varieties of different breeding periods
Purpose. To give comparative estimation of yield productivity and adaptability of spring barley varieties, developed at the V. M. Remeslo Institute of Wheat of NAAS and included to the State Register of Ukraine during 1...
Models of corn hybrids of different maturity groups FAO 150–490 for irrigated conditions
Purpose. To substantiate morphophysiological and heterozosis models of high yield hybrids of maize FAO 150–490 for irrigation conditions and to create appropriate genotypes with specific adaptability to agroecological fa...
Structural peculiarities of shoots of switch grass (Panicum virgatum L in the context of introduction in the Right-Bank Forest-Steppe and Polissia zones of Ukraine
Purpose. To define morphological peculiarities of the different types of shoots of switch grass (Panicum virgatum L.) in the context of introduction in the Right-Bank Forest-Steppe and Polissia zones of Ukraine. Methods...
Determination of commercially valuable characteristics of plant varieties for energetic use during the state examination
The analysis of commercially valuable indices of plant varieties for energetic use was carried out and the necessity to determine energetic indices during the state scientific-and-technical examination is substantiated....
Solving the issues in studying the soil cover at the institutions for plant varieties examination
Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling a...