Occurrence of Enteric Pathogens on Fresh Produce Grown on Irrigated Soils
Journal Title: Microbiology Research Journal International - Year 2015, Vol 6, Issue 1
Abstract
Aims: To assess the potential health risks of fresh produce grown on irrigated soils treated with manure in Kano State, a large produce region in Nigeria. Methodology: Fresh produce irrigated with wastewater on manure treated soils were assessed for the prevalence, serotype distribution and toxigenicity of Escherichia coli O157, Salmonella spp and Vibrio cholerae in a large produce region in Nigeria. A total of 230 samples obtained from five designated produce locations were examined using selective isolation method with prior enrichment. Fresh produce comprised carrots, cabbage, cucumber, lettuce, tomatoes, spinach, green peas and spring onions. Suspect isolates were identified and characterized by conventional biochemical methods and Microbact 24E (Oxoid, UK) kit. Confirmed isolates were serotyped and E. coli O157 and Vibrio cholerae O1 were assayed for their toxigenic potentials using the Reverse Passive Latex Agglutination kit. The enterotoxigenicity of Salmonella spp was determined by detection of stn gene using polymerase chain reaction (PCR) techniques. Results: Results obtained showed that overall, Salmonella spp and Escherichia coli O157 had the highest prevalence of 17.0% and 10.9%, respectively. Both were most commonly detected from fresh produce. The serotypes of Salmonella detected include Salmonella typhi (51.3%), Salmonella paratyhpi (20.5%) and Salmonella typhimurium (28.2%); strains of Vibrio cholerae O1 detected include Vibrio cholerae O1 of the Ogawa, Inaba and the Hikojima serotypes. Conclusion: The use of untreated irrigation water in vegetable production represents a significant route of transmission of diarrheal pathogen to humans and hence represents a public health risk. We recommend proper and adequate wastewater treatment before use.
Authors and Affiliations
G. O. Abakpa, V. J. Umoh, J. B. Ameh, S. E. Yakubu, J. K. P. Kwaga, A. M. Ibekwe
Keywords
Related Articles
- EP ID EP354693
- DOI 10.9734/BMRJ/2015/13910
- Views 55
- Downloads 0
Detection of Virulence Genes in Staphylococcus aureus and Streptococcus agalactiae Isolated from Mastitis in the Middle East
Aims: The current study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) and Streptococcus agalactiae (S. agalactiae) isolated from clinical and subclincal cases of bovine masti...
Species Distribution and Drug Susceptibility of Candida Isolates from Various Clinical Specimens at a Tertiary Care Hospital in Kashmir
Aims: The aim of our study was to identify the distribution of Candida species among clinical isolates and their sensitivity pattern for common antifungal drugs. Study Design: Prospective observational study. Place and D...
Bacteriological and Pathological Studies of Mammary Glands Affections in Camels (Camelus dromedarius) at Tumbool Abattoir, Sudan
Aims of the Study: To isolate and identify the bacteria associated with mastitis and study the pathology of udder tissue. Study Design: Out of 353 culled she-camels (from Tumbool abattoir), 105 (29, 7%) sections of udder...
The Effects of Different Storage Temperatures on the Microbial, Physicochemical and Organoleptic Quality Changes in the Shellfish "Ngolo" (Thais califera) from Nigeria
Aims: To investigate the effects of different storage temperatures (-15±2°C; 4±2°C and 29±2°C) on quality changes in ‘Ngolo’ (Thais califera) to evaluate the potential enhancement or otherwise of its domestic and global...
Molecular Epidemiology of Foot-and-Mouth Disease Viruses Circulated in Bangladesh from 2011–2014
Background: Foot-and-mouth disease (FMD) is one of the highly contagious and economically devastating diseases of cloven-hoofed animals in Bangladesh. The present study was undertaken to investigate the molecular epidemi...