Occurrence of enterotoxigenic Staphylococcus aureus in food.
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2002, Vol 9, Issue 2
Abstract
Gastroenteritis is one of the most frequent microbial diseases, which is caused by the ingestion of food contaminated with staphylococcal enterotoxins. In our study, the production of staphylococcal enterotoxins A, B (SEA, SEB) and the presence of respective staphylococcal enterotoxin genes were investigated in the field S. aureus isolates obtained from foods and food industry manufactures in East Slovakia. Radioimmunoassay (RIA), polymerase chain reaction (PCR) and dot-blot hybridisation were used for examination. The ability to synthesise enterotoxins was found in 20 (39.2%) of the total number of 51 isolates. Production of SEA was recorded in 3 (5.9%), production of SEB in 12 (23.5%) and production SEA together with SEB in 5 (9.8%) staphylococcal isolates. Nine (47.4%) sheep cheese isolates of the total number of 19 produced enterotoxins, especially SEB (36.8%). S. aureus isolates from pasta were enterotoxigenic in 6 cases (33.3%). The synthesis of enterotoxins was not detected in Bryndza cheese and sausages isolates. One enterotoxigenic isolate was obtained from smears of technological equipment and 4 isolates from throat and nasal swabs. No differences in results were recorded between RIA and PCR as well as PCR and dot-blot hybridisation. Our results suggest that it is of special importance to follow the presence of enterotoxigenic S. aureus strains in foodstuffs, especially for protecting the consumers from food poisoning.
Authors and Affiliations
Beata Holecková, Emil Holoda, Marián Fotta, Viera Kalinácova, Július Gondol', Ján Grolmus
Keywords
Related Articles
- EP ID EP58745
- DOI -
- Views 157
- Downloads 0
Pro-health behaviours – a sense of coherence as the key to a healthy lifestyle in rural areas?
[b][/b][b]Background. [/b]Health-related behaviours and lifestyle are related to the salutogenic concept of health, whereas the major public health problems faced in rural areas include difficulties in encouraging people...
Addison’s disease due to tuberculosis of the adrenal glands
Introduction: Addison’s disease (AD), or primary adrenocortical insufficiency, was first described by Thomas Addison in patients with adrenal tuberculosis (TBC). Over the past several decades, along with the introduction...
A small-scale survey of hantavirus in mammals from eastern Poland
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to floodin...
Proliferation of cells and expression of RARs, RXRs and HPV viral E6 and E7 proteins in cervical cancer cell lines after treatment with ATRA.
All-trans retinoic acid (ATRA) is considered to be a potential chemotherapeutic drug due to its capability to regulate cell growth and differentiation. The effects of ATRA on the proliferation of cells and gene regulatio...
Epidemiological assessment of maxillofacial fractures in inhibitants of Lower Silesia, Poland, in 2002–2006 – Treatment of maxillofacial fracture
[b]Introduction and objective[/b]. Epidemiological assessment of maxillofacial fractures carried out by a given specialized centre may constitute valuable material for comparison with other regions of Poland and the Euro...