Occurrence of Pathogenic and Multidrug Resistant Salmonella spp. in Poultry Slaughter-House in Bangladesh
Journal Title: Bioresearch Communications - Year 2018, Vol 4, Issue 2
Abstract
Salmonella is a diverse food-borne and zoonotic pathogen. The route of transmission is cardinal for surveillance and epidemiological investigations. The present study was conducted to characterize circulatory genotypes and pathogenicity of MDR Salmonella spp. from poultry slaughter house. Three swab samples from slaughterer's hand and poultry residual container were collected from a slaughter-house of Dhaka city. Of these, 7 isolates were confirmed as Salmonella spp. by phenotypic and molecular analysis. All seven isolates showed positive result for invA genes. Virulent genes (stn and sopB)specific PCRpresented13.3% and 6.66% positive isolates, respectively. Genotyping using Random Amplified Polymorphic DNA (RAPD) analysis exhibited six genotypes. Phylogenetic analysis and sequencing from each genotype exhibited close similarity to Salmonella enterica, S. Anatum and S. bongori from the slaughter hand and container swab. Antibiogram profiling presented 100% resistance to ampicillin and 83.33% to imipenem. This study recommends that dispersion of MDR and pathogenic Salmonella spp. from poultry slaughter house may pose threat to public health if goes unchecked.
Prevalence of fungi associated with Bipolaris leaf blight (BpLB) of different wheat varieties in Bangladesh
Thirty five fungal species, representing 20 genera were found to be associated with BpLB infected leaves of twenty one wheat varieties, collected from eight districts (Dhaka, Gazipur, Dinajpur, Joypurhat, Pabna, Sirajgon...
Identification of compound heterozygosity for a rare beta-globin gene mutation, codon 15 (-T) with a common mutation IVS1-5 (G>C) by direct sequencing in a Bangladeshi patient.
Most of the beta-thalassemia cases in Bangladesh are caused by 5-7 common mutations in the beta-globin gene. The present study reports a rare mutation in compound heterozygosity with a common mutation in beta-globin gene...
Isolation, Purification and Characterization of an Extracellular Protease from a Locally Isolated Bacillus sphaericus SI-1
An extracellular protease was purified from the culture supernatant of Bacillus Sphaericus strain SI1 isolated from local soil sample. The enzyme purification was primarily performed by ammonium sulfate fractionation, ce...
In Silico Computational Prediction of Anti-Breast Cancer Effect of Abruquinones from Abrus precatorius L.
Knowledge based searching of phytochemicals with potential anti-breast cancer effect from Abrus precatorius (L) with prediction of mechanism of action using computational molecular docking approach was the aim of this in...
Isolation of Bioactive Principles and Studies of Antimicrobial, Cytotoxic and Antioxidant Activities of the stem bark of Baccaurea ramiflora (Euphorbiaceae
Phytochemicals are extensively found at different levels in many medicinal plants. Stem bark of Baccaurea ramiflora (common name Latkan) belonging to the family of Euphorbiaceae, has been studied for isolation of its sec...