Ocena ekspresji markerów apoptozy w ziarninie w przewlekłym zapaleniu ucha środkowego
Journal Title: Otolaryngologia Polska - Year 2009, Vol 63, Issue 2
Abstract
Introduction: There is a growing evidence that molecular and cellular mechanisms may play a role in pathogenesis in chronic otitis media. The aim of the study: was to determine the intensity of apoptosis in granulation tissue in chronic otitis media. Material and method: Fifty four patients with chronic otitis media, who underwent surgical treatment, were enrolled into the study. The apoptosis was measured in paraffi n-embedded granulation tissue specimens by an immunohistochemical methods, by staining with a monoclonal antibody against apo-1/Fas/CD95 and P53 protein. The bacteriological evaluation of middle ear discharges were also done. Results: It was found statistically significant difference in expression of apo-1/Fas antigen between the groups with good clinical course (good healing and without recurrence) than those in the group with poor healing and recurrence (mean percentage of immunopositive cells 1,52 vs 3,34 respectively, p<0,001). The activity of apo-1/Fas antigen was more intense in tissue samples from the group with bacterial infection caused by Pseudomonas aeruginosa/Proteus sp/Staphyloccocus MRSA than those in the group without this infection (mean percentage of immunopositive cells 3,78 vs 1,75 respectively, p<0,001). The differences were also observed for P53 protein expression between the same groups, however they were not signifi cant. There was no differences between the groups of patients with granulomatous and cholesteatomatous chronic otitis media. The signifi cant negative correlation was found between expression of apo-1/Fas antigen and expression of P53 protein (r= - 0,64, p<0,001). Conclusions: In granulation tissue in chronic otitis media different expression of apo-1/Fas antigen was found in relationship to clinical course of disease and bacterial infection caused by Pseudomonas aeruginosa/Proteus sp/Staphyloccocus MRSA. It may suggest that apoptosis mediated by apo-1/Fas mechanism may contribute to pathogenesis of chronic otitis media.
Authors and Affiliations
Anna Pajor, Marian Danilewicz, Tomasz Durko, Andrzej Jankowski
Keywords
Related Articles
- EP ID EP51703
- DOI -
- Views 120
- Downloads 0
Complete traumatic laryngotracheal disraption
SUMMARY Closed injuries with complete separation larynx and tracheae are very rare. They are connected with high risk of death in the place of an accident. It is connected with acute respiratory obstruction due to local...
Zmiany impedancji elektrod u użytkowników implantów ślimakowych
SUMMARY Impedance measurements are routinely performed during the cochlear implan-tation. These measurements make possible to check, whether all electrodes work correctly and are useful in the monitoring of implant's fun...
Nagła głuchota spowodowana uderzeniem pioruna
The authors present a case of 12-years old girl with sudden sensorineural hearing loss caused by computer adapter explosion during the storm. She denied any tinnitus and vertigo. She was admitted immediately to ENT Ward....
Ekspresja genów w raku płaskonabłonkowym krtani in vivo na podstawie analizy mikromacierzy oligonukleotydowych
Streszczenie rozprawy na stopień doktora habilitowanego nauk medycz-nych nadanego przez Radę Wydziału Lekarskiego Śląskiego Uniwersytetu Medycznego w Katowicach w dniu 8.04.2010 r
Activity of vestibular organs in women with postmenopausal osteoporosis
Osteoporosis is a systemic disease characterized by low bone mass and alterations in the microstructure of the skeleton. It is a worldwide disease, most prevalent in women over the age of 70. It is estimated that approxi...