On the function of pentadecanoic acid and docosahexaenoic acid during culturing of the thraustochytrid, Aurantiochytriumsp. NB6-3
Journal Title: Journal of Biochemical & Microbial Technology - Year 2015, Vol 3, Issue 1
Abstract
During culturing of Aurantiochytriumsp NB6-3, the maximum triglyceride (TG) content was obtained at the end of logarithmic phase, after which it rapidly decreased until the mid-stationary phase. Of these TGs, pentadecanoic acid (C15), palmitic acid (C16) and docosahexanoic acid (DHA) were major fatty acids. Interestingly, the unusual fatty acid-C15 reached a maximum content of 22mg/g lyophilized cells at the end of the logarithmic phase, after which it decreased to 6 mg/g cells at the end of the culture. The variation in the C16 content was similar to that in C15 content. On the other hand, the variation in DHA content followed the trend of C15 until 60 h, then reached a minimum value at 72 h, after which it increased again to finally reach to up to 30 mg/g cells at the end of the culture. To better understand the function of C15 and DHA in TG, Aurantiochytriumsp NB6-3 was cultured in growth media containing fatty acid mixture of DHA and C15, DHA and myristic acid (C14), C15 and oleic acids (C18=1), or C14 and C18=1, respectively. In C15 and/or DHA-containing media, the cell growth was stimulated. The cell growth was most stimulated in growth media containing both DHA and C15. When BalB/3T3 mouse fibroblasts were cultured with mixtures of C15 and DHA or mixtures of C18=1 and C15, similar results were obtained. These results showed that DHA and C15 synergistically stimulated the cell growth of Aurantiochytriumsp. and mouse fibroblasts.
Authors and Affiliations
Kaya K, Ikeda K, Kose R, Sakakura Y, Sano T
Keywords
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- EP ID EP567599
- DOI 10.14312/2053-2482.2015-1
- Views 85
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