Ontogeny and induction of digestive enzymes in Scylla serrata larvae fed live or artificial feeds or their combination
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2012, Vol 5, Issue 3
Abstract
Generally, the patterns of digestive enzyme activities during ontogeny and the digestive capacities of most crab larvae are less understood. In the present study, ontogenetic patterns of enzyme activities of amylase, trypsin and leucine aminopeptidase (LAP) were investigated for the first time in developing Scylla serrata larvae. Moreover, the influence of diet types (live feed, artificial feed or combination of both feeds) on larval digestive enzyme activities was also examined. Results showed that amylase activity were low at early larval stages zoea 1 (Z1) to zoea 3 (Z3), exhibited enzyme activity peaks at stages zoea 4 (Z4) and zoea 5 (Z5) and declined gradually at megalopa to crab instar 1(C1) stage. Trypsin showed an elevated activity at Z2, remained low at later stages but enzyme activity rose again from megalopa until reaching the highest activity at the C1 stage. In contrast, LAP enzyme activities increased gradually from Z3, reaching a maximum at the C1 stage. Factorial ANOVA showed that enzyme activities were influenced by the developmental stage, by the type of diet, and by the interaction of these factors. Also, the use of Discriminant Analysis (DA) allowed us to show that there was a significant effect of the diet as well as development stage on digestive enzyme activities. Furthermore, DA allowed us to rank the parameters that weighted mostly in the model: trypsin>LAP>amylase in discriminating between development stages while only LAP was the predictor variable for discriminating between diets.
Authors and Affiliations
Augusto Serrano, Rex Traifalgar
Keywords
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