Optimization of Alcalase hydrolysis conditions for production of Dagaa (Rastrineobola argentea) Protein hydrolysate with antioxidative properties
Journal Title: Industrial Chemistry - Year 2017, Vol 3, Issue 1
Abstract
Enzymatic conditions; stirring speed, pH, temperature, enzyme substrate (ES) ratio, solvent ratio, reaction time; for Dagaa (Rastrineobola argentea) hydrolysis by Alcalase 2.4L® were optimized. This was done to guarantee maximum enzyme activity, minimized losses and subsequent maximized product yield. This study showed optimized hydrolysis conditions to be fixed at minimum stirring speed (overhead stirrer, Stuart, UK), pH 7, 56°C, ES ratio of 2% (v/w) and solvent ratio of 0.5% (v/w) for 6 h. At these combined optimal conditions, Alcalase 2.4L®Dagaa (Rastrineobola argentea) hydrolysis resulted in 71% protein recovery with 83% degree of hydrolysis. These findings substantiated the use of Dagaa which proved to be a relatively good substrate for protein hydrolysate production.
Authors and Affiliations
Ogonda Lydia Awuor
Keywords
Related Articles
- EP ID EP356264
- DOI 10.4172/2469-9764.1000122
- Views 15
- Downloads 0
Optimization of Alcalase hydrolysis conditions for production of Dagaa (Rastrineobola argentea) Protein hydrolysate with antioxidative properties
Enzymatic conditions; stirring speed, pH, temperature, enzyme substrate (ES) ratio, solvent ratio, reaction time; for Dagaa (Rastrineobola argentea) hydrolysis by Alcalase 2.4L® were optimized. This was done to guarantee...
Process Simulation for Industrial Process Design
Process design and optimisation are fundamental steps to ensure the economic sustainability of chemical processes. Suitable description of the performance of the various unit operations of the plant should be made and in...
Kinetics, Optimization and Proximate Analysis of Drying Moringa Oleifera Seeds in A Tray Dryer
This study examined the drying behaviour, optimum drying conditions and proximate analysis of Moringa oleifera seeds in a tray dryer. Thirteen (13) experimental data sets were generated using Box-Behnken design of design...