Optimization of callus induction and cell suspension cultures of Catharanthus roseus
Journal Title: Journal of Herbal Drugs - Year 2014, Vol 5, Issue 3
Abstract
Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids. Vincristine and vinblastine; two anti-microtubular agents of vinca alkaloids are the first natural drugs utilized in cancer therapy. Monomeric alkaloids such as vindoline and catharanthin are precuser for other alkolids. These reasons are motives to its production by tissue culture. The goal of this investigation is to characterize the best level plant hormones in the in vitro culture. Experimental: Improvement experiment of production callus carried out in Factorial arrangement with three agents, Explant, amount of plant hormones (2, 4-D, and BAP). Comparison mean showed leave explants had callus in number of treatments but these callus were strong and stem explants had medium callus production in more treatment than leave explants. For improvement suspension culture used of Randomized complete with six treatment of plant hormones (2, 4-D, BAP, and KN) in three repeats. Results & Discussion: Number of the cell and dried weight cell measured for mean comparison. Variance analysis number of cells showed that had significant difference in p>0.01 and second treatment produced most cell numbers and so in dried weight cells had significant difference in p>0.01 and third treat produced most biomass in all treatments. Recommended applications/industries: Results of this study indicated that 2.5 mg/L 2, 4-D and 2 mg/L BAP are suitable for suspension cell culture and callus production in Catharanthus roseus.
Authors and Affiliations
Ziba Fooladvand; Bahman Fazeli Nasab; Reza Derikvand; Abdolah Ghasemi Pirbaloti
Keywords
Related Articles
- EP ID EP23457
- DOI -
- Views 283
- Downloads 18
Collection and identification of medicinal plants used by the indigenous people of Mobarakeh (Isfahan), southwestern Iran
Background & Aim: Knowledge of the traditional application of plants is declining faster than the plants themselves; this study was conducted to record and store the local knowledge and cultural outcomes for using the me...
Seed germination and growth improvement in Heracleum persicum Desf. by osmo-primming
Background & Aim: Because of seed germination ability of medicinal plants seed is low and, generally exposed to problem. Priming is one of the techniques that help seeds to be germinated faster and uniformly....
Cytogenetic study of two Alhagi species
Background & Aim: Alhagi genus has two important medicinal plant species in Iran, including Alhagi pseudoalhagi, and A. graecorum (known “Kharshotor” in Persian). These plants are distributed in Iran like weeds. General...
Chemical composition of the essential oils Ziziphora clinopodioides collected from Tuyserkan (Hamadan), Iran
Background & Aim: Ziziphora clinopodioides Lam (Kakuty-e-Kohi) is an aromatic and medicinal plant belonging to the family Labiaceae. The essential from this species contains pulegune that having effects such as sedative,...
Determine optimum of harvest time on the quantity/quality of essential oil and thymol of thyme (Thymus vulgaris L.) in Isfahan
Background & Aim: Thyme is common named Thymus vulgaris L. belongs to Lamiaceae family. Thyme is a most important medicinal plant that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed...