Optimization of Carbon Sources for the Amylase Production and Growth of Bacillus licheniformis JAR-26 under Submerged Fermentation
Journal Title: Indian Journal of Biology - Year 2017, Vol 4, Issue 1
Abstract
Amylases (EC 3.2.1.1, 1, 4--D-glucan glucanohydrolase) are one of the most important and oldest industrial enzymes that hydrolyze starch at -1, 4 glycosidic bond in the interior of the starch molecule, and hold the maximum market share of enzyme sales. Amylases are ubiquitously produced by plants, animals and microorganisms, however, microbial sources are the most preferred for large scale production and industrial use. The production of - amylases from microbes depends on the strain, physical (pH, temperature, aeration) and nutritional (carbon, nitrogen, mineral ions) factors. Keeping this in view, the present study aimed to investigate effect of different concentrations (control, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0% w/V) of five carbon sources viz starch, glucose, maltose, fructose and sucrose on growth and enzyme production from B. licheniformis JAR-26. Among the tested carbon sources, maltose proved best carbon source for amylase production and maximum amylase production was recorded at 2% maltose (4.181 U/ ml of medium). At this concentration growth/OD was 1.529 and bacteria could utilize 98.2% of the available sugar in the medium. After maltose, starch was second suitable source for enzyme production and 2% starch showed 3.622 U/ml enzyme production. Glucose and fructose resulted in higher biomass yield (maximum biomass at 2% glucose, OD=1.741) in comparison to other sources but amylase production was very poor (lowest at 4% fructose, 0.710 U/ml). From comparison of the various treatments, it is suggested that for maximum biomass production of B. licheniformis JAR-26, growth medium may be supplemented with 3% glucose whereas to achieve maximum amylase production culture medium may be supplemented with 2% maltose under submerged fermentation.
Authors and Affiliations
Nand Lal
Keywords
Related Articles
- EP ID EP452105
- DOI 10.21088/ijb.2394.1391.4117.5
- Views 108
- Downloads 0
Necessity of Ecological Balance for Widespread Biodiversity
The ecological balance is a stable state between all plants and animals in an ecosystem and destabilization of this stable state is ecological imbalance that in turn exerts serious threats to widespread nature of biodive...
Clinico-Pathological analysis of Hb S/β+ Th Patients in Odisha: A Tertiary Care Hospital Based Study
India is ethnically a diverse country with marked regional variation. Due to migration, there is constant mixing of people from different regions. These migrations not only helped in creating variations leading to positi...
Biodeterioration of Cultural Heritage and Indigenous Methods Used for Preserving Cultural Heritage
The world is full of cultural heritage of all kinds. A large number of monuments, artefacts and manuscripts spread all over the world are finest example of rich cultural heritage and a symbol of men’s cultural identity a...
Developement of Yield Predictive Models on the Basis of Thermal Indices for Brassica Juncea in Tarai Region of Uttranchal
A field investigation was carried put during rabi season of the year 2014 and 2015 at Norman E. Borlaug Crop Research Centre (CRC) of the Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, (Uttarakh...
Effect of Different Visible Light Wavelengths on Seed Germination and Photosynthetic Pigment Contents in Vigna unguiculata (L.) Walp
Among the various naturally occurring abiotic factors regulating plant development, light (radiations) plays an important role in photosynthesis, photoperiodism and photomorphogenesis. The present investigations aimed to...