OPTIMIZATION OF CAROTENOID PRODUCTION BY PARACOCCUS BEIBUENSIS USING PLACKETT-BURMAN DESIGN AND RESPONSE SURFACE TECHNIQUE.
Journal Title: PARIPEX-Indian Journal of Research - Year 2017, Vol 6, Issue 7
Abstract
This work is aimed at evaluating the conditions for growth and carotenoids production by Paracoccus beibuensis. The sequential statistical methods were used to maximize carotenoid production. Initially, a Plackett-Burman design was used to optimize the variable i.e. nutritional components such as glucose, peptone, beef extract, meat extract and NaCl, out of which meat extract effects significantly for carotenoid production (P < 0.05). Further investigation of effect of meat extract using a Response Surface Methodology (second -order central composite design (CCD) with one factor analysis) was done to optimize carotenoid production, which was adequately approximated with a full quadratic equation obtained from a two-factor-2-level design. The 2 analysis of quadratic surfaces showed that meat extract has significant effect on carotenoid production (P < 0.05) (R ) 0.93 and adjusted R-squared 0.90. Validation of the experimental model was done where maximum carotenoid production (1112μg/l) was obtained with 15g/l of meat extract. The carotenoid production is more sensitive for presence of meat extract and its concentration. Maximum biomass production was achieved with 15g/l of meat extract.
Authors and Affiliations
Deepti D. Dhere, S. M. Dharmadhikari
Keywords
Related Articles
- EP ID EP557242
- DOI -
- Views 89
- Downloads 0
A STUDY ON KNOWLEDGE, ATTITUDE AND PRACTICE OF MEDICAL ETHICS AMONG INTERNS AND POSTGRADUATES IN MEDICAL COLLEGE, BENGALURU
Background: The rise in the legal confrontations between patients and doctors has become an issue of concern in recent times. Ethics teaching has been shown to have a significant influence on the professionalism and mora...
AWARENESS, PRACTICE, MYTHS, FACTS AND IMPACT OF HEALTH EDUCATION IN DENGUE CONTROL IN A RURAL POPULATION
Introduction: Prevention of breeding of Aedes mosquito is an effective method for dengue control. Practice of source reduction to prevent breeding depends on the knowledge of the community, which can be impr...
Independent predictors of coronary artery disease through computed tomography coronary angiography
Objective: To investigate the presence coronary artery lesions, atherosclerotic plaque characteristics, coronary artery disease (CAD) extension and coronary artery calcium score in normotensive and hypertensive patients,...
ATTITUDE TOWARDS CAREER COUNSELING SERVICES AMONG STUDENTS IN SELECTED UNIVERSITIES IN KENYA
This paper discusses the attitude students have towards Career Counselling services in Kenyan universities including its usefulness in preparing graduates for the job market. In an attempt to examine this, a sample size...
TREATMENT OF WASTE WATER USING CONSTRUCTED WETLANDS
Natural wetlands are being considered beneficial for food and habitat for wildlife, water quality improvement, flood protection and shoreline erosion control. There is increasing advocacy by environmental management scho...