Optimization of ChIP protocol for investigation of canonical Wnt target genes expression in new born heart
Journal Title: Фактори експериментальної еволюції організмів - Year 2018, Vol 22, Issue
Abstract
Aim. In present work we have focused on chromatinimmunoprecipitation (ChIP) protocol with next qPCR optimization for analysis of canonical Wnt target genes regulation by b‑catenin and plakoglobin in new born heart. Methods. In study we have used the new born hearts tissue at the age of 3 days after birth (P3). We have analyzed hearts of control mice and mice with heterozygous knockout of β-catenin. We have proceeded ChIP protocol based on standard protocols in our modifications. ChIP results validated with qPCR. Results. We have adopted ChIP protocol for investigation of signalling function of β-catenin and plakoglobin as well as for identification of β-catenin and plakoglobin specific binding genomic region in new born heart. Conclusions. We have shown that plakoglobin is able to compensate the signaling function of β-catenin and binds with similar genomic region under β-catenin haploinsufficiency conditions. Keywords: β-catenin, plakoglobin, Wnt signaling, gene expression, heart, chromatinimmunoprecipitation, ChIP.
Authors and Affiliations
О. О. Півень
Keywords
Related Articles
- EP ID EP618745
- DOI -
- Views 48
- Downloads 0
Генетичний потенціал бактерій, що виділені із забруднених кадмієм грунтів
Мета. Визначення таксономічного положення та стійкості виділених із забруднених кадмієм ґрунтів бактеріальних культур до іонів токсичних металів – Cd2+, Cu2+, Ni2+, Co2+, CrO42-. Методи. Культури бактерій виділяли методо...
Использование ДНК-маркеров для изучения генетического полиморфизма генотипов мягкой пшеницы (Triticum aestivum Desf.) Азербайджана
Цель. Работа посвящена молекулярно-генетической оценке генотипов 110 образцов мягкой пшеницы из различных регионов Азербайджана. Методы. Молекулярно-генетический. Результаты. С применением 8 ISSR маркеров уровень генетич...
Influence of various factors on the antiradical activity of Panax ginseng С. A. Meyer extracts
Aim. The aim of the work was to evaluate the anti-radical activity of extracts of ginseng's underground and overground organs Panax ginseng C.A. Mey, depending on various factors – age of plants, collection time and cult...
The effect of various NaCl concentrations on morphophysiological properties of bread wheat sprouts (Triticum aestivum L.)
Aim. The effects of various NaCl concentrations on germination energy, germination percentage of seeds and morpho-physiological indices of seedlings have been determined in bread wheat (Triticum aestivum L.) varieties. M...
Dependence of grain productivity of the main shoot ear on the stem dry matter weight in varieties of winter wheat
Aim. Estimation of the mass of dry matter of the stem of the main shoots of different varieties of winter wheat in the phases of flowering and full ripeness as a criterion for the grain productivity of its ear in years w...