Optimization of Conditions for in vitro Production of Fructooligosaccride using Aureobasidium pullullans
Journal Title: Scholars Academic Journal of Biosciences - Year 2015, Vol 3, Issue 1
Abstract
Abstract: Oligosaccharides are very well recognized as ‘functional food ingredients’ because of their positive effects on human health. This research work focuses on the microbial production of Fructosyl Transferase (FTase) and the production of Fructooligosaccharides (FOS) by transfructosylation using this enzyme. Fructooligosaccharides are industrially produced from sucrose by microbial enzymes with transfructosylating activity. Fructosyltransferase is produced intra- and extracellulraly by several microorganisms including bacteria and fungi. Despite the large number of microbial FTase producers, only a few of them have the potential for industrial application and were focused in several studies about FOS production. The increasing interest in prebiotic compounds opens also possibilities for small-scale use of FTase. In the present study fungus Aureobasidium pullulans was used to produce FOS and effect of different physical and chemical parameters on FOS production were studied. The analysis of the product was performed by High Pressure Liquid Chromatography (HPLC). Keywords: Fructooligosaccharide, FOS, Fructosyl transferase enzyme, HPLC, Probiotics, Aureobasidium pullulans
Authors and Affiliations
Anupam Mandal, Sanjeev Ranjan, Imran Ansari
Keywords
Related Articles
- EP ID EP377939
- DOI -
- Views 95
- Downloads 0
Sclerotinia Rot of Ridge Gourd and Pointed Gourd in Lateritic Zone of West Bengal, India
Abstract: Sclerotinia rot caused by Sclerotinia sclerotiorum (Lib.) de Bary was recorded on ridge gourd (Luffa acutangula (L.) Roxb.) and pointed gourd (Trichosanthes dioica Roxb.) in Birbhum district of West Bengal. The...
Third molar as a diagnostic tool for age estimation in forensic odontology: An exploratory study
Age estimation is an essential part of forensic expertise. Identifying individuals in cases of natural disasters, wars, criminal cases and in terms of employment also, age estimation is an important tool. Apart from the...
Culturable Diversity of Thermophilic Microorganisms Found in Hot Springs of Northern Himalayas and to Explore Their Potential for Production of Industrially Important Enzymes
Thermophiles are the microorganisms that live and grow in extremely hot environment that would kill most other microorganisms. They can be isolated from a number of marine and terrestrial geothermally heated habitats inc...
A Study of Derived Savanna environment through airborne palynomorphs, Anyigba, Kogi State, Nigeria.
A study of airborne palynomorphs of Derived Savanna environment, Nigeria during the dry season (October-December) and wet season (April-June) in 2012, revealed the presence of great diversity of palynomorphs consisting o...
Tumors of the Spinal Cord: Histopathological and Radiological Correlation with Review of Literature
Primary spinal cord tumors constitute 10% to 15% of all primary central nervous system tumors. Lesion outside the dura are termed extradural and lesions within dura are called intradural. Intradural lesions are divided...