Optimization of culture conditions for high frequency in vitro shoot multiplication in sugarcane (Saccharum officinarum L.)
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 3
Abstract
Present study deals with the optimization of various culture conditions for initiating high frequency in vitro shoot multiplication in two early maturing high yielding sugarcane genotypes namely Co98014 & Co89003. On the behalf of the findings of this study, it was concluded that the temperature, photoperiod and culture media pH affected the frequency of in vitro shoot multiplication in both sugarcane genotypes at a significant level. In both genotypes high frequency shoot multiplication was recorded at growth room temperature 25ºC, 16h/8h light/dark photoperiod and culture media pH 6.0. Genotype Co89003 exhibited highest shoot regeneration and multiplication under various culture conditions. The present study suggests the necessity of investigation of these culture conditions separately upon individual sugarcane genotypes prior to develop efficient in vitro plant regeneration protocol for commercial purposes.
Authors and Affiliations
M. K. Sharma, R. S. Senger, P. S. Chand, R. Singh, S. Gupta, A. Singh
Keywords
Related Articles
- EP ID EP285974
- DOI -
- Views 54
- Downloads 0
Oil content and fatty acid composition of soybean (Glysine max L.) genotypes evaluated under rainfed conditions of Kashmir Himalayas in India
Soybean (Glycine max), a multipurpose crop with much appreciated nutritional and functional properties, has generated a lot of interest during last few decades for improving its cultivation and characteristics. Besides h...
Studies on site specific nutrient management (SSNM) on hybrid sunflower seed production in Southern Karnataka
A field experiment was conducted at the Main Research Station, University of Agricultural Sciences, Hebbal, Bangalore, to know the effect of Site Specific Nutrient Management (SSNM) on growth (plant height (cm), Leaf are...
Potential of Inula racemosa root extract and its fractions to suppress root-knot nematode Meloidogyne incognita
Nematicidal potential of chloroform root extract of Inula racemosa and its fractions was investigated on egg hatching and mortality of root knot nematode Meloidogyne incognita. Egg masses and second stage juveniles (J2)...
Behavioural shift of estuarine mudcrab as biomarker of arsenic exposure in Sundarbans estuary of West Bengal
Mudcrab Scylla serrata (Crustacea: Decapoda) in an ecologically and economically important species of Sundarbans Biosphere Reserve was studied for its behaviour under the exposure of toxic arsenic - a common xenobiotic o...
Vegetation indices mapping for Bhiwani district of Haryana (India) through LANDSAT-7ETM+ and remote sensing techniques
This study describes the VIs Vegetation Condition Index in term of vegetation health of wheat crop; with help of LANDSAT-7ETM+ data based NDVI and LAI for Bhiwani District of Haryana states (India) and gave the spatial d...