Optimization of DNA Extraction and PCR Conditions for Genetic Diversity Study in Artemisia Herba-Alba from Algeria
Journal Title: Scientific Journal of Genetics and Gene Therapy - Year 2016, Vol 2, Issue 1
Abstract
Background: Artemisia herba alba is an aromatic species very rich in secondary metabolites that can be used in traditional medicine. The context and purpose of the study: This species suffers from the degradation of its natural habitat. The creation of collections containing diverse ecotypes is important for its preservation. This species contains essential oils which limit the extraction of DNA. The objective of this study is to optimize the DNA extraction and PCR conditions. The method will be used to evaluate the genetic diversity of Artemisia herba alba in the highlands of Western Algeria as a starting point. Results and main findings: The use of different reagents and the modification of the incubation time allow obtaining white and clear DNA. Moreover, the modification of temperature and duration of hybridization results in clearer ISSR profiles. The use of the genetic markers for the analysis of genetic material ISSR, carried out on 12 individuals, indicates that A. herba alba is characterized by a broad genetic polymorphism.
Authors and Affiliations
Maghni Benchohra, Bougoutaia Youcef, Abderrabi Khadidja, Adda Ahmed, Merah Othmane
Keywords
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- EP ID EP555570
- DOI 10.17352/sjggt.000008
- Views 35
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