Optimization of Growth Conditions for Spawn Production of Pleurotus Florida
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2019, Vol 9, Issue 1
Abstract
Gilled mushrooms are widely accepted as the edible mushroom. Oyster Mushroom or Pleurotus Florida are commonly cultivated around the world. Fungi(Pleurotus) showed dits role as my co-remediation for pollutants degradation similar to polycyclic aromatic hydrocarbons and petroleum. Spawns are the seed extracted for growing mushrooms; they are vegetative mycelium of oyster mushroom cultured in Potato Dextrose Agar medium with grain as secondary food or Nutrition such as wheat, Black Gram, Maize, Pea grains etc. However, spawn are grains covered with mushroom mycelium. An effective result was obtained at Potato Dextrose Agar media with fresh mycelium and High yield grains of Wheat. Bottle of Spawn filled with Boiled wheat’s up to 70% along with 5% of the mycelium of test oyster mushroom. Conditions allotted included sterilization with UV for one hour and autoclaving of Bottles for my celium inoculation followed by maintaining the temperature of 20⁰ C for 20 days to obtain Mother culture. Subsequent transfer of 30% of mother spawns to boiled wheat grains for 15 days incubation results in sister culture and 11 days for planting culture.
Authors and Affiliations
Virendra Pandey, Sourabh Singh, Vivek Agnihotri, Arpit Shrivastava, Deepak Mishra
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