Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 7
Abstract
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concentration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3- methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida: Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X1 2 – 3.210 X3 2 – 6.786 X4 2 – 7.290 X1 2 X3 + 5.575 X1X3 2 – 4.843 X3 2 X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.172 5 mg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.
Authors and Affiliations
Islamudin Ahmad, Arry Y Yanuar, Kamarza Mulia, Abdul Mun'im
Keywords
Related Articles
- EP ID EP269178
- DOI 10.1016/j.apjtb.2017.06.010
- Views 47
- Downloads 0
Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens
Objective: To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods: Two independent experiments were conducted. In Experiment 1, total IgA levels were quantifi...
Anticancer activity of Salvia officinalis essential oil and its principal constituents against hormone-dependent tumour cells
Objective: To investigate the in vitro antiproliferative action of essential oil from Salvia officinalis L. (S. officinalis) grown in Sicily (Italy), and its main components on hormone-dependent cancer cell lines. Method...
Effects of black chokeberry extracts on metastasis and cell-cycle arrest in SK-Hep1 human liver cancer cell line
Objective: To determine the anti-cancer properties of black chokeberry extract on the SKHep1 human liver cancer cell line. Methods: MTT cell proliferation assay, wound migration, invasion, zymography and cell cycle were...
Science behind cisplatin-induced nephrotoxicity in humans: A clinical study
Objective To investigate the relationship between serum electrolyte changes and cisplatin induced nephrotoxicity. Methods We collected data from 18 patients undergoing cisplatin chemotherapy including serum electrolyt...
In vivo anticancer activity of vanillin semicarbazone
Objective To evaluate the anticancer activity of vanillin semicarbazone (VSC) against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice. Methods The compound VSC at three doses (5, 7.5 and 10 mg/kg i.p.) was...