Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 7
Abstract
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concentration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3- methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida: Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X1 2 – 3.210 X3 2 – 6.786 X4 2 – 7.290 X1 2 X3 + 5.575 X1X3 2 – 4.843 X3 2 X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.172 5 mg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.
Authors and Affiliations
Islamudin Ahmad, Arry Y Yanuar, Kamarza Mulia, Abdul Mun'im
Anticancer activity of Salvia officinalis essential oil and its principal constituents against hormone-dependent tumour cells
Objective: To investigate the in vitro antiproliferative action of essential oil from Salvia officinalis L. (S. officinalis) grown in Sicily (Italy), and its main components on hormone-dependent cancer cell lines. Method...
New Thai herbal shampoos as pediculicides for killing head louse, Pediculus humanus capitis De Geer (Phthiraptera)
Objective: To evaluate the efficacy of Thai herbal shampoos [Cartoxylum formosum (C. formosum) + eucalyptus essential oil (EO), C. formosum + citrus EO, Solanum trilobatum + eucalyptus EO, Solanum trilobatum + citrus EO,...
Antihyperglycemic effect of methanol extract of Tamarix aphylla L. Karst (Saltcedar) in streptozocin–nicotinamide induced diabetic rats
Objective: To evaluate the antihyperglycemic potential of Tamarix aphylla (T. aphylla) leaves in STZ–NIC induced diabetes in Wister Albino rats. Methods: Acute toxicity study was done to check the toxicity of T. aphylla...
Antibacterial activity of naringenin-rich fraction of pigeon pea leaves toward Salmonella thypi
Objective: To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi). Methods: The leaf sample was powdered and macerated with methanol and fractioned by liquid–liquid...
Antidiarrhoeal activity of leaf methanolic extract of Rauwolfia serpentina
Objective To evaluate the antidiarrhoeal property of methanol extract of the leaves of Rauwolfia serpentina (R. serpentina) in experimental diarrhoea induced by castor oil in mice. Methods Doses of 100, 200 and 400 mg...