Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 7
Abstract
Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concentration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3- methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida: Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X1 2 – 3.210 X3 2 – 6.786 X4 2 – 7.290 X1 2 X3 + 5.575 X1X3 2 – 4.843 X3 2 X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.172 5 mg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.
Authors and Affiliations
Islamudin Ahmad, Arry Y Yanuar, Kamarza Mulia, Abdul Mun'im
Diospyros lycioides Desf.: Review of its botany, medicinal uses, pharmacological activities and phytochemistry
Diospyros lycioides Desf. (D. lycioides) is traditionally used as herbal medicine against various human and animal ailments in tropical Africa. The present paper reviewed information on botany, medicinal uses, phytochemi...
Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae)
Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high p...
Antioxidant activity, total phenolic, and resveratrol content in five cultivars of peanut sprouts
Objective: To investigate the change in total phenolic compounds, antioxidant activity, and resveratrol content of five different germinated peanut cultivars. Methods: The germinated sprouts of five peanut cultivars (Kal...
Incidence and histopathology of encysted progenetic metacercaria of Clinostomum complanatum (Digenea: Clinostomidae) in Channa punctatus and its development in experimental host
Objective To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum (C. complanatum) in Channa punctatus (C. punctatus), associated histopathology and the experimental infection to laborator...
Clinical features of severe malaria: Protective effect of mixed plasmodial malaria
Objective: To investigate clinically severe malaria patients with Plasmodium falciparum (P. falciparum), Plasmodium vivax (P. vivax) and mixed species infections. Methods: This study was conducted at Dr. Saiful Anwar Gen...