OPTIMIZATION OF LACTOBACILLUS ACIDOPHILUS 55 CULTIVATION IN A MEDIUM WITH SODIUM SELENITE
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 2
Abstract
Aim. Optimization of culture conditions of the probiotic strain Lactobacillus acidophilus 55 in the medium with sodium selenite. Methods. Studies of the influence of sodium selenite, inoculum size and cultivation time on the concentration of viable cells of L. acidophilus 55 were performed using a three-level factorial experiment on Box-Behnken (BBD). Results. It has been found that the inoculum size is the factor that has the greatest effect on the concentration of viable cells. The optimum conditions to maximize the concentration of viable cells in the presence of selenium ions were: the content of sodium selenite in the medium of 8.3%, the amount of inoculum 5.3% and 18 hours of cultivation time. Conclusions. When L. acidophilus 55 was cultured at optimized conditions, the highest concentration of viable cells was 2.5×108 CFU/ml.
Authors and Affiliations
K. S. Ogirchuk, N. K. Kovаlenko, O. A. Poltavska
Keywords
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- EP ID EP395493
- DOI 10.18524/2307-4663.2014.2(26).48257
- Views 82
- Downloads 0
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