Optimization of large-scale production process for human prothrombin complex concentrates
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 12
Abstract
Objective To determine the optimal process conditions for efficiently extracting human prothrombin complex concentrates from human plasma. Methods Using human plasma as the materials and the yield of prothrombin complex concentrates as the evaluation standard, the preparation process parameters were studied and optimized through design of exporement(DOE), orthogonal experiments, and single factor experiments. Results The optimal process conditions were as follows: DEAE Sephadex A50 gel was selected, which balanced to pH 7.6, and then the amount of 1.7-2.5 g/L of plasma weight is added into the cryoprecipitate supernatant for adsorption for 40 minutes; Washing solution (0.15-0.175 mol/L sodium chloride) with 3 times the volume of gel was washed 3 times, and eluent (0.5-2.0 mol/L sodium chloride) was washed 3 to 5 times; Add stabilizer (heparin 35 IU, sodium chloride 0.1 mol/L) for ultrafiltration dialysis. Conclusion By using the optimized process mentioned above, the yield(measured by human coagulation factor IX)can reach 620 000 to 630 000 IU/ton of plasma, which is suitable for large-scale production.
Authors and Affiliations
Liangchao ZHAO, Guanjun LI, Xiaowei MA, Dongsheng ZHAO, Minghua WNAG, Bo SONG
Keywords
Related Articles
- EP ID EP738724
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.12.015
- Views 40
- Downloads 0
Changes of blood routine parameters in elderly apheresis platelet donors
Objective To analyze the changes of blood routine and platelet related parameters of the elderly apheresis platelet donors since the first donation, so as to ensure the health and platelet quality of the elderly apheresi...
Construction of a rapid depression screening model for blood donors in Guangzhou based on decision tree
Objective To investigate the prevalence of depression in blood donors and analyze the related factors, so as to develop a rapid depression screening model for blood donors. Methods A total of 13 015 street whole blood do...
Efficacy of apheresis and concentrated platelets under different transfusion strategies
Objective To retrospectively analyze the clinical effects of apheresis and concentrated platelets under different transfusion strategies, so as to explore more scientific transfusion strategies. Methods A total of 279 pa...
Drug resistance mutation analysis of HIV-1 among blood donors in Dalian: 2011—2020
Objective To study the distribution and trend of HIV-1 drug resistance mutation in Dalian blood donors between 2011 and 2020. Methods The protease-reverse transcriptase (PR-RT) region was sequenced in Dalian blood donors...
Advances in the application of lyophilized platelets and derivative products
Platelets are important components of the blood system. There are many kinds of concentrated platelets and their derivatives, among which platelet-rich plasma (PRP ), growth factors (GFs) and platelet-rich fibrin (PRF) h...