Optimization of Media Formulations for Callus Induction, Shoot Regeneration and Root Induction in Nicotiana benthamiana
Journal Title: Journal of Plant Science and Research - Year 2016, Vol 3, Issue 1
Abstract
Nicotiana tabacum and N. benthamiana have served as model species for the plant sciences for many years. For the studies involving transformation analysis using Agrobacterium, Nicotiana benthamiana has been gaining importance in recent years due to its ability to express auto fluorescent protein for detection of transformed genes and its efficiency in gene expression compared to other plants. This study aims at optimizing the culture media procedures for the in vitro culturing of this model plant. BAP (1 mg/l), IAA (0.1 mg/l) was suitable for callus induction in 20 days. This result was in accordance with previous studies. BAP (2 mg/l), IAA (1 mg/l), Kinetin (0.5 mg/l) was suitable for shoot regeneration in 25 days. This result differed from that of previous studies where BAP (1 mg/l) and NAA (0.1 mg/l) was used for both callus induction and shoot regeneration. Thus, this study forms the basis for invitro rising of Nicotiana benthamiana for transformation and genetic engineering studies.
Authors and Affiliations
Geethalakshmi Sundararaman
Keywords
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