OPTIMIZATION OF NUTRIENT MEDIUM COMPOSITION FOR CULTIVATION OF BACILLUS THURINGIENSIS 87/3 ENTOMOPATHOGENS
Journal Title: Подільський вісник : сільське господарство, техніка, економіка - Year 2018, Vol 1, Issue 28
Abstract
The aim of the research was to develop an optimal liquid medium composition for bacteria strain B. thuringiensis 87/3 which is the most favorable for the identifiying the potential production of biologically active components. Nutrient medium for cultivation of Bt 87/3 was optimized base on a full-fledged experiment, according to the Box-Behnken (33) plan. The influence of different medium composition on the growth of microorganisms was investigated during active experiment process. The nutrient medium for cultivation was optimized by the composition of carbon and nitrogen feed, as well as on the content of micro elements. To increase the yield of heat-resistant spores and the amount of endotoxin, the nutrient medium was enriched with corn extract, amino acids and mineral salts (Mg, Mn, diammonium phosphate). The rational limits and the corresponding optimal values of the substances (factors) that make the nutrient medium are determined. Optimum concentrations were investigated in the medium of carbon source (15 g/l of glucose), nitrogen (10 g/l of corn extract) and content of phosphorus-containing inorganic salts (1,5 g/l of diamonium phosphate). When growing this strain in periodic conditions at 30°C, the number of viable cells reached 4,4×109 cells/ml, while synchronous spore formation was observed. The optimal component variant is at the intersection of the maximum value of the desirability function in the specified interval of each factor. In this case, rational limits and corresponding optimal values factors that make up the B. thuringiensis nutrient medium are determined. Nutrient medium that was optimized can be recommended for cultivation of Bacillus thuringiensis 87/3 in laboratory and production conditions.
Authors and Affiliations
М. V. Boiko
Keywords
Related Articles
- EP ID EP523287
- DOI -
- Views 114
- Downloads 0
RESEARCH ON INFLUENCE OF ELECTROMAGNETIC RADIATION ON ENDOMETRITIS TREATMENT IN DAIRY COWS
According to literary sources from 14% to 40% of cows are ill in postpartum endometritis, and at some farms this number is up to 90% of cows. Endometritis causes risks of fertilization failure from 17% to 40%, increasing...
METROLOGICAL ENSURING CONTROL OF ELECTRICAL PARAMETERS OF DRINKING WATER
Currently, the problem of drinking water quality is important. A promising trend in the industry is controlling the electrical parameters of drinking water (admittance). It correlates with indicators of quality non-elect...
REASONING OF THE MAIN CONSTITUENTS OF ECONOMIC MECHANISM OF STIMULATION OF PRODUCTION OF ENVIRONMENTALLY FRIENDLY AGRICULTURAL PRODUCTION
The unsystematic irrational use of land resources in the conditions of absence of clear ecological and economic reasoning of the prospects for the development of the agrarian sector leads to the loss of agricultural land...
FRAGMENTAL COMPOSITION OF WATER IN LEAVES OF POTATO VARIETIES (SOLANUM TUBEROSUM L.)
The high yield is explained by the saturation of the soil with water; therefore, the study of indicators of providing plants with water during the growing season is an important area of research in modern agrarian scienc...
PROCESS OF INTERWORKING RELATIONSHIP BETWEEN KEYBAR AND SOIL IN TERMS OF MULTISTAGE PLOWING
The author examines the interaction between keybar and soil in terms of multistage plowing. The dependence of the split off soil layer length on the cutting angle and keybar running depth in terms of various depths of ti...