Optimization of Somatic Embryogenesis in Ferula assa-foetida L. (a Rare Medicinal Plant)
Journal Title: Annual Research & Review in Biology - Year 2015, Vol 5, Issue 5
Abstract
Experiments was carried out to examine the effect of abscisic acid (ABA), source of carbon and temperature on secondary somatic embryogenesis and somatic embryo germination of Ferula assa-foetida L. Cotyledonary somatic embryos were cultured on MS (Murashig and Skoog, 1962) medium supplemented with 0, 1.0, 1.5 or 2.0 mg/l ABA and on MS medium with 3% mannitol, sorbitol or sources. The highest number of secondary somatic embryos (35.94) was observed on MS medium devoid of ABA at 25°C, but the addition of abscisic acid, sorbitol or manitol to the MS basal medium significantly reduced the formation of secondary somatic embryos at 5°C. The concentration of abscisic acid had a significant effect on the rate of secondary somatic embryogenesis. Among the carbon source studied, mannitol significantly suppressed the induction of secondary somatic embryos. Abscisic acid had a positive effect on the percentage of somatic embryo germination. Higher germination was observed with1.0 or 1.5 mg/l ABA, but it was not significantly affected by sorbitol or manitol. Abscisic acid increased germination at 15°C and 25°C while sorbitol and manitol enhanced it at 5°C.
Authors and Affiliations
Mahmoud Otroshy, Shirin Roozbeh
Keywords
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- EP ID EP353688
- DOI 10.9734/ARRB/2015/12110
- Views 87
- Downloads 0
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