Optimization of the Cultural Parameters for Improved Production of Antimicrobial Metabolites by Streptomyces gulbargensis DAS 131
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 9
Abstract
Aims: To investigate the influence of appropriate culture medium by optimizing the cultural conditions affecting the growth and bioactive metabolite production by Streptomyces gulbargensis DAS 131 under submerged culture conditions in order to reduce the cost of fermentation process to improve the formation of antimicrobial compounds. Place and Duration of Study: Department of Botany and Microbiology, January 2012 to May 2012. Methodology: The impact of environmental parameters such as incubation period, pH, temperature and salt concentration and effect of various nutrients such as carbon and nitrogen sources and minerals on the antimicrobial metabolite production by Streptomyces gulbargensis DAS 131 was evaluated by employing agar well diffusion assay. Growth was measured in the form of dry mycelial weight. Results: The optimum pH and temperature for bioactive metabolite production were 7 and 35°C respectively. Highest antimicrobial metabolite production was found when the strain was inoculated into the medium amended with glucose at the concentration of 2%, soya peptone at the rate of 1% and NaCl at the concentration of 5% and incubated for six days under shaking conditions. The metabolites showed good antimicrobial activity against Gram positive and Gram negative bacteria, as well as unicellular and multicellular fungi. Conclusion: S. gulbargensis DAS 131 isolated from the semi-arid soils of Gulbarga, Northern Karnataka province, India exhibited broad spectrum antimicrobial activity. It was found that the antimicrobial metabolite production by the strain was positively influenced by carbohydrates, nitrogen sources and minerals.
Authors and Affiliations
Usha Kiranmayi Mangamuri, Muvva Vijayalakshmi, Sudhakar Poda, Dayanand Agasar
Keywords
Related Articles
- EP ID EP344925
- DOI 10.9734/BJPR/2014/7085
- Views 76
- Downloads 0
Antioxidant and Cytotoxic Activity of Crude Flavonoid Fraction from the Fruits of Hybrid Variety of Momordica charantia (Bitter Gourd)
Aims: The study was conducted to evaluate the antioxidant and cytotoxic properties of crude flavonoid fraction from hybrid variety of Momordica charantia (L.) (Bitter gourd) fruit. Place and Duration of Study: The study...
Pharmacological Activities of Epithio Steroids
The present review describes biological activities of semi- and synthetic epithio steroids. About fifty biologically active compounds have shown confirmed antitumor, immunosuppressant, or aromatase inhibition and other a...
Fed Bioequivalence Studies for Immediate Release Drug Products: Beneficial or Wastage?
Aims: The present study was undertaken to understand the need of fed bioequivalence studies for immediate release pharmaceutical products and thereby evaluating the relative appropriateness of guidelines given by the Eur...
Effect of Vitellaria paradoxa Stem Bark Ingestion on Kidney Functions in Wistar Rats
Background: Vitellaria paradoxa is used in the treatment of different diseases in several parts of Nigeria without regard for its safety. Aim: This study was aimed at investigating the effect of ingestion of Vitellaria...
Simultaneous Spectrophotometric Determination of Diflunisal and Diclofenac Sodium in Pharmaceutical Dosage Form Using Chemometric Methods
Aims: Is the simultaneous determination of Diflunisal and Diclofenac sodium in pharmaceutical preparations. Study Design: Four spectrophotometric methods (Classical Least Squares, Iterative Target Transformation Factor...