Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production
Journal Title: Biotecnología Vegetal - Year 2020, Vol 20, Issue 3
Abstract
In Cuba, a biofertilizer has been developed based on a nitrogen fixer Azotobacter chroococcum strain. This work aimed to optimize the culture medium and the fermentation conditions for industrial scale. First, tests in the shaker were carried out and then in a fermenter. In each case an experimental validation was performed. For the optimization of the culture medium, a central composite design was used to adjust response surfaces. A 23 factorial design with equidistant star points and six repetitions in the center of the plan. A total of 19 experimental runs were performed, with three repetitions determined. As a response variable, cell growth was determined by optical density λ = 540 nm (OD540). To optimize microbial growth based on agitation speed and air flow, a 7.5, liter with a factorial design 22 was used, taking OD540 as the response variable. With the means of optimized cultivation a growth of 2-4 x 109 UFC ml-1 was obtained that allow to increase the content of bacterial biomass together to the use of a speed of agitation and good flow of air. The new conditions allowed to reduce the time of fermentation at 10 hours, less than 20 to 22 hours used in the production scheme. In addition, 40%, 50% and 20% respectively, of carbon, nitrogen and phosphorus sources were reduced.
Authors and Affiliations
María Cristina Pérez Peñaranda, Julio Oramas García, Emilio Arcadio Sotolongo Valdés, Yoandra Román Tabio Román Tabio, Héctor López Rodriguez
Keywords
Related Articles
- EP ID EP697682
- DOI -
- Views 69
- Downloads 0
Response of Phaseolus vulgaris L. cultivars to drought stress
Tolerance to drought is a desired cultivars of Phaseolus vulgaris L. The present study aimed to determine the response of 22 cultivars of common bean during the early stages of vegetative development character. The pla...
Novel explant for somatic embryogenesis in Sorghum bicolor (L.) Mohen
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations o...
In vitro establishment of Handroanthus heptaphyllus nodal segments
Micropropagation could be used as a viable alternative in the mass production of Handroanthus heptaphyllus (Vell.) Mattos (white lapacho). The objective of the present work was to establish in vitro of nodal segments of...
Determination of the content of the main components of the culture medium during the differentiation phase of cell suspensions of Coffea arabica cv. Catimor 9722
Exhaustion of culture medium components and possible influence on the in vitro development of plants has been little studied. The current work was developed in order to know and determine contents of the main component...
Development of adventitious buds in banana (Musa sp.) Cv. Big Dwarf (AAA)
Several cytokinins were studied in the culture media with the aim of induction of adventitious buds in the cv. Gran Naine. The largest explant values with development of adventitious buds were obtained when Thidiazuron...