Optimization of the new Plates-B&T method for nucleated cell separation in cord blood
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 7
Abstract
Objective To optimize the bottom and top(B&T) method for nucleated cell separation in cord blood in order to reduce the disturbance and improve the nucleated cell yield. Methods A new device comprised of two plastic plates and connection units was developed. B&T Cord blood bag was put between the plates, which were then centrifuged and put into the serum expresser together to go through serum and red blood cells separation simultaneously. After centrifugation parameter adjustment, the new Plates-B&T method was compared with traditional method in parallel. Results New Plates-B&T method could avoid buffy coat movement and disturbance. Compared to conventional PRP method, Plates-B&T method reduced centrifugation and separation time from(29.12±2.79)min to(14.91±2.12)min(P<0.05). Using new method, the recovery rate of total nucleated cell(TNC) was (89.04±5.19)%, the removal rate of red blood cell(RBC) was(55.56±22.57) %, and hematocrit(HCT) in buffy coat was(0.48±0.09)L/L. RBC removal was enhanced significantly, especially for cord blood samples with medium-low volume. Conclusion Based on B&T method, we established a new device and method, which increased the efficiency and quality of cord blood nucleated cell separation.
Authors and Affiliations
Chunyan WU, Jie ZHANG, Shunji XIE, Mi QIU, Qiang CHEN, Zhenyang LAI
Keywords
Related Articles
- EP ID EP738299
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.07.019
- Views 40
- Downloads 0
Investigation of coordinated development between blood banks and apheresis plasma collection banks
Objective To explore the factors that affect the coordinated development of blood banks and apheresis plasma collection banks (hereinafter referred to as plasma banks), and explore feasible measures for the coordinated d...
Research progress on platelet rich plasma in management of patients with knee osteoarthritis
Knee osteoarthritis (KOA) is a common chronic degenerative disease of cartilage, which often occurs in middle-aged and elderly patients. It can cause joint swelling, pain and limited movement. With the aging population g...
Molecular analysis of the new allele 803delC of subtype B
Objective To analyze the serological characteristics and molecular mechanism of a novel B subtype allele 803delC. Methods ABO blood group was detected by serological method. Sequence-specific primer polymerase chain reac...
Establishment of platelet donor gene bank involving specific antigen HPA 1-6/10/15/21 by real-time fluorescent PCR
Objective To research the genetic polymorphism of HPA 1-6/10/15/21 in platelet donors in Zhongshan area, and establish a gene bank of platelet donors with HPA locus. Methods The HPA 1-6/10/15/21 system genotyping was per...
Determination of suspended erythrocyte volume range and establishment of its internal control standard
Objective To determine the volume range of suspended erythrocyte and establish its internal control standard. Methods The theoretical value of suspended erythrocyte volume was calculated according to the screening criter...