Optimization of β-galactosidase Production from Yogurt and Dairy Soil Associated Bacteria Using Different Fermentation Media
Journal Title: Microbiology Research Journal International - Year 2016, Vol 11, Issue 2
Abstract
Aims: The aim of current research was the production of β-galactosidase enzyme from Lactobacillus delbruekii subsp. bulgaricus isolated form yogurt and soil samples. Study Design: Production of β-galactosidase. Place and Duration of Study: Microbiology laboratory, Department of Zoology, GC University, Lahore, Pakistan, between 14 Nov, 2013 and 29 Nov, 2014. Methodology: Bacterial isolates were isolated from yogurt and soil samples. Morphological, biochemical characterization, and 16S rRNA sequencing was used for the identification of the bacterial strains. The optimization and maximum production of β-galactosidase was carried out by using various temperatures, pH, carbon sources (corn flour, wheat flour, rice flour), nitrogen sources (Trypton, Peptone, Yeast extract), and incubation periods. Results: National Centre for Biotechnology Information (NCBI) Blast analyzed that A11, A13 and A14 strains belongs to Lactobacillus delbruekii subsp. bulgaricus with accession numbers KP256199, KP264120, and KP264121. Maximum enzyme production was obtained after 48 h of incubation at 40-45°C temperature at pH 6.0-7.0. The highest enzyme production was observed in the presence of rice flour and yeast extract. The maximum enzyme activity was shown by A11, A13 and A25 (7.69 U/mL, 7.25 U/mL, and 7.28 U/mL). Conclusion: Rice flour could be the potential carbon source for the production of β-galactosidase from Lactobacillus delbruekii subsp. bulgaricus whereas nitrogen sources reduced its production. Temperature and pH was important physical parameter for the production of β-galactosidase from yogurt and soil associated bacteria.
Authors and Affiliations
Nazish Mazhar Ali, Saiqa Andleeb, Bushra Mazhar, Agha Usman Ali Khan
Keywords
Related Articles
- EP ID EP350747
- DOI 10.9734/BMRJ/2016/18750
- Views 83
- Downloads 0
Evaluation of C19 Steroid Intermediates during Microbial Transformation of Phytosterol
Aim: To assay for C19 steroid intermediates during biotransformation of phytosterol. Study of Design: Pretreatment and processing of wheat bran and rice bran, Sulphate-phosphate-ferric technique, phytosterol screening, f...
Diversity of Rotavirus VP7 and VP4 Genotypes Associated with Severe Childhood Diarrhea in North West Cameroon: Detection of Unusual Strains G1P[6], G2P[6], G2P[8] and G3P[6]
Aims: To determine rotavirus genotypes and distribution among children 0 - 5 years old with severe acute diarrhea in the North West Region (NWR) of Cameroon. Study Design: Cross sectional. Place and Duration of Study: H...
Virulence Profile, Fluoroquinolone and Quinolone Resistance of Uropathogenic Escherichia coli Isolates Recovered from Thabet Hospital-Tulkarm, Palestine
Backcground: Escherichia coli (E. coli) is the most common cause of urinary tract infection (UTI). Virulence factors are mainly responsible for the severity of these emerging infections. Aims: To analyze virulence factor...
Serum Levels of Immunoglobulin A, G & M in Chlamydia trachomatis Infection among Primary and Secondary Infertility Patients
Background: Chlamydia trachomatis is a well- known sexually transmitted bacteria that is capable of damaging female reproductive tract leading to infertility. Chlamydia trachomatis infection in men can mechanically hinde...
Antibiotic Susceptibility Test of Klebsiella pneumoniae and K. oxytoca Isolated from Different Clinical Samples and Perform Random Amplified Polymorphic DNA among K. pneumoniae
Aims: To study the prevalence of MDR and ESBL producing Klebsiella pneumoniae and Klebsiella oxytoca and perform RAPD (Random amplified polymorphic DNA) by optimizing PCR among isolated ESBL producing Klebsiella pneumoni...