Optimized combinations of statins and azoles against Acanthamoeba trophozoites and cysts in vitro
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2019, Vol 12, Issue 6
Abstract
Objective: To evaluate the combination of several statins (atorvastatin, fluvastatin and simvastatin) and azoles (voriconazole, posaconazole and itraconazole) against Acanthamoeba spp. Methods: The efficiency of the different drug combinations against the trophozoite stage of different Acanthamoeba strains were evaluated by Alamar Blue assay. Effect on the cyst stage was observed by inverted microscope. Cytotoxicity of combinations of azoles and statins was evaluated by measuring the release of lactate dehydrogenase from a murine macrophage cell line. Results: Combinations of any of the tested statins and voriconazole or posaconazole were more efficient in inhibiting Acanthamoeba compared to statins or azoles individually. The drug combinations at the combined inhibitory concentrations 50% showed lower toxicity compared to that of the compounds alone. Conclusions: The combinations of statins together with voriconazole and posaconazole are more efficient than these drugs alone, and these combinations have lower cytotoxicity in mammalian cell lines.
Authors and Affiliations
José E. Piñero
Keywords
Related Articles
- EP ID EP594006
- DOI 10.4103/1995-7645.261287
- Views 62
- Downloads 0
Tropical herbs and spices from Mauritius as alternative biomedicine to manage communicable and non-communicable diseases
Mauritius, is a famous touristic tropical island that forms part of the Mascarene Islands in the Indian Ocean. The Island is also well known for its rich cultural and ethnic diversity and for its singular flora and fauna...
Annona muricata: Is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects
Annona muricata (A. muricata) is a tropical plant species belonging to family Annonaceae and known for its many medicinal uses. This review focuses on the research history of its traditional uses, phytochemicals, pharmac...
Steroidal alkaloids ameliorate cell proliferation, oxidative stress, inflammation and histology outcome in vitro and in vivo
Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-α-induced human RA-FLS M...
Epidemiology and immunodiagnostics of Strongyloides stercoralis infections among migrant workers in Malaysia
Objective: To investigate the status of Strongyloides(S.) stercoralis infections among migrant workers in Malaysia for the first time and identify risk factors. Methods: Four diagnostic methods were employed for the dete...
The Belt and Road Initiative: Challenges and opportunities in tackling emerging infectious diseases
The majority of emerging infectious disease agents affecting human are RNA viruses that originate from animals. Globalization and climate changes continue to reshape the geographical distribution of humans, animals, vect...