Optimizing C:N Ratio, C:P Ratio, and pH for Biosurfactant Production by Pseudomonas fluorescens
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 7, Issue 2
Abstract
Aim: To optimize the combination of selected culture medium parameters so as to achieve maximum biosurfactant production from Pseudomonas fluorescens. Study Design: (1) Culturing P. fluorescens for biosurfactant production using a glycerol-mineral salt media with variations in ratio, carbon and phosphorus ratio (C:P ratio), and pH, (2) Screening for biosurfactant activity, (3) use of Response Surface Methodology in determining the combination of the factors that will lead to maximum biosurfactant production. Place and Duration of Study: Department of Microbiology, Faculty of Science, University of Port Harcourt, Nigeria, between September 2016 and June 2017. Methodology: Pseudomonas fluorescens was cultured for biosurfactant production using glycerol-mineral salt media with variations in carbon and nitrogen ratio(C:N ratio), C:P ratio, and pH. Combination of these factors was optimized via the use of Response Surface Methodology. The range of values of the factors investigated was C:N: 20–60, C:P: 10–16, pH: 5.5–8.5. Fifteen experimental runs were carried out. Results: At the end of the experimental runs, the surface tensions of the culture broths ranged from 30.66 – 51.90 mN.m-1. The surface tensions were fitted into the generalized polynomial model for 3-factor design. The model was worked out to be Y = 296.5533 – 34.4456X1 + 0.602833X2 – 23.4019X3 – 0.0545 X1X2 + 1.088889X1X3 + 0.020417 X2X3 + 1.663333X12 – 0.00596X22 + 0.551944X32. Prediction profiles generated from this model showed that the lowest surface tension, indicating maximum biosurfactant production, was achievable at a combination of pH 5.5, C:N = 20, and C:P = 16. Use of this combination for biosurfactant production resulted in reduction of the surface tension of the broth culture from 60.04 mN.m-1 to 30.64 mN.m-1. This almost tallied with the predicted value of 30.57 mN.m-1. Conclusion: The optimized combination avoided wastage of the carbon source. It is thus economical to carry out optimization procedures before proceeding to commercial production of biosurfactants.
Authors and Affiliations
P. L. Peekate, G. O. Abu
Keywords
Related Articles
- EP ID EP338300
- DOI 10.9734/JAMB/2017/38199
- Views 75
- Downloads 0
Solid Substrate Fermentation of African Bush Mango (Irvingia gabonensis) Seeds
Aim: This study investigated the effect of fermentation on nutrient and antinutrient contents of African bush mango (Irvingia gabonensis). Methodology: Bush mango seeds were fermented for a period of five days. Microbiol...
Yield of Metarhizium anisopliae Conidia on Four Tropical Cereals and in vitro Evaluation of Virulence against Cowpea Weevil, Callosobruchus maculatus (Fabricius) (Coleoptera: Chrisomelidae)
Aims: To evaluate conidia yield of entomopathogenic fungus, Metarhizium anisopliae Net 275.86DC on different tropical cereal substrates and evaluate its virulence against the bruchid weevil, Callosobruchus maculatus in v...
Immunomodulatory and Toxicological Effect of Goya Extra Virgin Olive Oil in Albino Rat Orogastrically Dosed with Salmonella typhi
The immunomodulatory and toxicological properties of Goya extra virgin olive oil on albino rats orogastrically dosed with Salmonella typhi were evaluated in this study. Twenty albino rats were randomly distributed into f...
Bacterial Resistance to Antibiotics: Current Situation in Sudan
Aims: This work was conducted to evaluate the antimicrobial resistance of Gram positive and Gram negative bacteria of human and animal origin to commonly used antibiotics in Sudan according to the previous studies on ant...
Assessment of the Microbial Diversity of Spent-Oil Contaminated Soil in Calabar, Nigeria
This study was designed to assess the total heterotrophic and hydrocarbon oxidizing microbial diversity, determine the physicochemical parameters of oil contaminated soil and evaluate the potentials of microorganisms iso...