OPTIMUM CONDITIONS FOR CYTOSINE DEAMINASE PRODUCTION BY LOCAL ISOLATE OF ESCHERICHIA COLI E9.
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 12
Abstract
This study was aimed to isolate a higher cytosine deaminase producer Escherichia coli and studying the optimum condition for its production, a total of twenty-five urine samples were collected for the isolation of E. coli bacterium from Al-Imamein AlKadhumain medical city hospital in Baghdad. From these samples, a total of 10 bacterial isolates were obtained when subjected to morphological and microscopical tests. Cytosine deaminase enzyme activity was determined using cytosine as substrate, results indicated that 10 isolates of them are cytosine deaminase producer with different specific activity ranged between (0.122-0.35) U/mg and the isolate Escherichia coli E9 was the most efficient in the production of cytosine deaminase with specific activity of 0.35 U/mg protein. Therefore, it was chosen to determine the optimum conditions for cytosine deaminase production. Maximum cytosine deaminase production was achieved after supplementation of the minimal salt medium (pH 8.5) with 0.1% citric acid, 0.1% peptone and incubated at 37oC for 24h. Under these conditions, the specific activity of cytosine deaminase produced in culture supernatant was sharply increased to 0.7 U/mg protein.
Authors and Affiliations
Ali Saadi Al-Baer
Keywords
Related Articles
- EP ID EP674581
- DOI -
- Views 187
- Downloads 0
CARDIAC TROPONIN AND CREATINE KINASE IN HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENTS ON ANTIRETROVIRAL DRUGS
People living with HIV are living a longer and productive life due to the effective viral suppression by highly active anti retroviral therapy (HAART). They are therefore exposed to metabolic syndromes associated with ag...
COCOA MISTLETOE (LORANTHUS BENGWENSIS) AQUEOUS EXTRACT ATTENUATES DICLOFENAC INDUCED ORGANS TOXICITIES IN WISTAR ALBINO RATS
Mistletoe (Loranthus bengwensis) has been used as a therapeutic agent and also possesses some beneficial nutrients. This present study therefore investigate the antioxidant, antitoxicity and chemo-protective effects of a...
PROTECTIVE AND CURATIVE ACTIVITY OF ETHANOL LEAF EXTRACT OF CORCHORUS OLITORIUS IN THIOACETAMIDE EXPOSED RATS
The present study was undertaken to evaluate the protective and curative effect of ethanol leaf extract of Corchorus olitorius against thioacetamide-induced toxicity in experimental rats. The animals were exposed to thio...
PREVALENCE OF OBESITY AMONG SAMPLE OF COLLEGE STUDENTS
Background: Obesity and overweight are considered a public health problem due to their high prevalence in different age groups, mainly in young adults, as well as the short, medium, and long term consequences of obesity,...
POTENTIAL TEST AND COMPARATIVE TEST OF AMOXYCILLIN ANTIBIOTICS USED IN THE TREATMENT OF CASES OF RESISTANCE IN TASIKMALAYA, INDONESIA
Objective: ARI is an acute infection of any part of the respiratory tract including the paranasal sinuses, middle ear and pleural cavity caused by viruses, bacteria, rickettes. Amoxycillin is one of antibiotics to overco...