Organization of actin cytoskeleton in normal and regenerating arterial endothelial cells.
Journal Title: Proceedings of the National Academy of Sciences of the United States of America - Year 1983, Vol 80, Issue 8
Abstract
The distribution of actin stress fibers in normal and regenerating (after endothelial denudation by means of a balloon catheter) rabbit aortic endothelial cells has been studied by means of immunofluorescence with human actin autoantibodies on en face endothelial cell preparations. Our results show that: (i) under normal conditions actin is accumulated as a network at the periphery of endothelial cells. Stress fibers are present only in endothelial cells located immediately below intercostal artery branches; (ii) stress fibers develop in endothelial cells early during regeneration and persist after the end of endothelial mitotic and motile activities; and (iii) the orientation of stress fibers within the cytoplasm follows the direction of blood flow, with the exception of stress fibers situated in cells at the edge of the wound, when endothelial cell progression toward the denuded area as well as mitotic activity have ceased. We conclude that stress fibers are an organelle present in endothelial cells in vivo and that they reorganize during endothelial cell adaptation to unfavorable or pathological situations.
Authors and Affiliations
G Gabbiani, F Gabbiani, D Lombardi, S M Schwartz
Keywords
Related Articles
- EP ID EP82704
- DOI -
- Views 63
- Downloads 0
NMDA antagonist inhibits the extracellular signal-regulated kinase pathway and suppresses cancer growth.
Glutamate antagonists limit the growth of human cancers in vitro. The mechanism of anticancer action of NMDA antagonists is not known, however. In this article, we report that the NMDA antagonist dizocilpine inhibits the...
Macrophage migration inhibitory factor (MIF) plays a pivotal role in immunity against Salmonella typhimurium.
The cytokine macrophage migration inhibitory factor (MIF) exerts a multitude of biological functions. Notably, it induces inflammation at the interface between the immune system and the hypothalamus-pituitary-adrenal str...
Controlled proteolysis of the multifunctional protein that initiates pyrimidine biosynthesis in mammalian cells: evidence for discrete structural domains.
The multifunctional protein that initiates de novo pyrimidine biosynthesis in mammalian cells carries carbamoylphosphate synthetase, aspartate transcarbamylase (aspartate carbamoyltransferase), and dihydro-orotase activi...
Transcriptional regulation of hepatitis B virus by nuclear hormone receptors is a critical determinant of viral tropism.
Hepatotropism is a prominent feature of hepatitis B virus (HBV) infection. Cell lines of nonhepatic origin do not independently support HBV replication. Here, we show that the nuclear hormone receptors, hepatocyte nuclea...
Independent and combined analyses of sequences from all three genomic compartments converge on the root of flowering plant phylogeny.
Plant phylogenetic estimates are most likely to be reliable when congruent evidence is obtained independently from the mitochondrial, plastid, and nuclear genomes with all methods of analysis. Here, results are presented...