OVARIECTOMISED AND LONG TERM INDUCED (OLI) RAT MODEL FOR PRECLINICAL ASSESSMENT OF OSTEOPOROTIC THERAPEUTICS.
Journal Title: Journal of Drug Delivery and Therapeutics - Year 2017, Vol 7, Issue 2
Abstract
Osteoporosis affects millions of people, especially the aged population and numerous researchers focus on developing novel pharmacotherapeutic agents for the prevention and treatment of osteoporosis. Animal models have become an indispensable part of the pre-clinical assessment of osteoporotic therapeutics and rat models have been widely preferred. The majority of studies so far reported have used young or short-term induced rat models, but they do not mimic the pathological condition in senile osteoporosis. Hence this study has focused on developing an ovariectomised and long term induced (OLI) aged rat model for the preclinical assessment studies. Long term osteoporosis was induced in Wistar rats by ovariectomy followed by the maintenance period of 10 months. Histological evaluation and Micro-CT analysis substantiated the extensive bone loss in the OLI rats compared to Control and ovariectomised and short term induced (OSI) rat model. OLI rats also exhibited impairment in the healing of 3 x 1.5 mm femoral cortical bone defect even after 8 weeks of surgery. Histomorphometric analyses indicated that OLI rats exhibited a poor regeneration efficiency (RE) ratio of 0.67 ± 0.2 compared to 0.95 ± 0.1 in the Control group. Chronic bone loss in the OLI rats together with the impaired osteogenic efficacy has been successfully demonstrated which proposes OLI rat models to be utilized for investigating novel pharmacotherapy’s or novel biomaterials intended for osteoporotic applications.
Authors and Affiliations
Chandran Sunitha
Keywords
Related Articles
- EP ID EP294661
- DOI 10.22270/jddt.v7i2.1400
- Views 103
- Downloads 0
HYPOGLYCEMIC EFFECT OF POLYHERBAL FORMULATIONS IN ALLOXAN INDUCED DIABETIC MICE
Objective: The present work was executed to evaluate the antidiabetic potency of herbal preparation which was the combination of wheat germ oil, fresh juice of Coriander sativum and Aloe vera gel in normal and alloxan...
Biomedical Nanomaterials: A Short Insight of Applications
Nanotechnology is rapidly expanding area in the field of science that helps us to manipulate matter at the atomic and molecular level thus having varied and new properties with huge potential. It is the study of extremel...
PHENOLIC, FLAVONOID AND TANNIN CONTENT DETERMINATIONS AND IN-VITRO ANTI-OXIDANT ACTIVITY OF ROOT EXTRACTS OF SACCHARUM MUNJA ROXB.
Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as ageing, cancer, arthritis, liver injury and cardiac complications. The fact between anti-...
APPLICATION OF TAGUCHI ORTHOGONAL ARRAY DESIGN FOR OPTIMIZATION OF CHITOSAN NANOPARTICLES OF HYDROPHOBIC CARDIOVASCULAR DRUGS
Nanotechnology based drug delivery system have shown to improve solubility, rate of dissolution and oral bioavailability of poorly water soluble drugs. Present study is aimed to develop Nebivolol loaded chitosan nanopart...
FLOW AND COMPACTION PROPERTIES OF THE MODIFIED BIOMATERIAL POWDER DERIVED FROM THE TYMPANOTONUS FUSCATA SHELL
A modified biomaterial powder derived from Tympanotonus fuscata shell was evaluated for its flow and compaction properties towards possible application in the directly compressible technology of tablet production. The pu...