Over-Expression of Arabidopsis Phytochelatin Synthase (Atpcs1) Gene in Escherichia Coli Confers Enhanced Tolerance to Cadmium
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 4
Abstract
Phytochelatin synthesis (PCS), the key enzyme involved in heavy metal detoxification and accumulation has been studied extensively for the purpose of Phyto remediation. In this study, we have isolated the AtPCS1 gene from Arabidopsis thaliana, which is considered as a potentially accumulator of heavy metals. The 1458bp CDNA of AtPCS1 was isolated, cloned and sequenced. The deduced amino acid sequence of the C - terminal region of the AtPCS1 contained homologous regions of functional domains of metallothionein and thioredoxin. Heterologous expression of AtPCS1 gene in E. coli resulted in an enhanced accumulation and tolerance to heavy metal Cadmium. This enzyme has the potential to develop transgenic plants with enhanced tolerance to heavy metals for phytoremediation.
Authors and Affiliations
Srinivas Naik Kethavath, Swathi Bai Moodavath, Hanumalal Nunavath, Bukya Bhima
Keywords
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