Oxidation and peroxidation in the uvea of the rabbit eyes with experimental uveitis and ocular hypertension
Journal Title: Офтальмологический журнал - Year 2019, Vol 0, Issue 2
Abstract
Background. The role of metabolic changes in the anterior eye tissues in the pathogenesis of uveal inflammation in patients with elevated intraocular pressure has been studied poorly. Of particular interest, in this respect, are free radical mechanisms, which can be a trigger of oxidant stress and cause damage to cell membranes in ocular tissues. Purpose. To study the activity of pro-oxidant enzymes and levels of lipid peroxidation products in the uvea tissues in the rabbits with experimental anterior non-infectious uveitis against the background of ocular hypertension. Material and Methods. Forty-one rabbits were divided into 4 study groups. Group 1: 10 animals with experimental ocular hypertension; group 2: 10 animals with experimental allergic uveitis; group 3: 12 animals with ocular hypertension and allergic uveitis; and group 4: 9 intact animals serving as controls. To simulate ocular hypertension in the groups 1 and 3, the animals were made a single 0.1 ml injection of 0.3% carbomer into the anterior chamber. The tissues of the uvea and the aqueous humour were studied biochemically. We estimated the activity of pro-oxidant enzymes, NADPH oxidase and xanthine oxidase, and the content of lipid peroxides: malonic dialdehyde and diene conjugates. The data of the experimental studies were processed using parametric statistical tests with an SPSS package and Statistica 5.5 Results. The activity of pro-oxidants enzymes was increased in all uvea tissues in all study groups; the maximal NADPH oxidase and xanthine oxidase activity was in the animals with both ocular hypertension and uveitis. The NADPH oxidase and xanthine oxidase activity was increased by 51.1% and 63.9% (р<0.001), respectively, as compared with controls. Lipid peroxidation with accumulation of toxic products in the uvea and aqueous humour was noted both in the ocular hypertension-only and uveitis-only groups; however, the maximal values were in an uveitis model against the background of ocular hypertension, where malonic dialdehyde and diene conjugates were increased, respectively, by 67.0% and 54.3% (р<0.001) in the aqueous humour, and, respectively, by 93.1% and 69.1% (р<0.001) in the uvea tissues, compared with controls. Conclusions. Our findings reveal an important link in the pathogenic action of elevated IOP which burdens inflammation in the uveal tissues through the activation of oxidative and peroxidative processes. In addition, our findings support the assumption that primary high-pressure glaucoma can be a factor which worsens inflammation in the anterior eye.
Authors and Affiliations
I. Mikheitseva, N. Bondarenko, S. Kolomiichuk, T. Siroshtanenko
Keywords
Related Articles
- EP ID EP586881
- DOI 10.31288/oftalmolzh201925560
- Views 100
- Downloads 0
Optical features of light passing through refractive structures of the eye
The features of light refraction in the anterior segment of the eye and after dispersion on the posterior surface of the cornea are considered. It is shown that the cornea refracts the light as a weakly scattering lens....
A Case Report of Kussmaul-Maier Disease
Background: Kussmaul-Maier disease (also known as polyarteritis nodosa or PAN) is a systemic disorder characterized by destructive and proliferative changes in the walls of arteries (mainly muscular arteries and arteriol...
Predicting the development and progression of primary open-angle glaucoma based on the determination of GST gene polymorphisms
Background: Epidemiological studies have demonstrated that primary open-angle glaucoma (POAG) is a complex multifactorial disease resulting from interaction of risk factors and gene polymorphisms. Previously, we have dem...
Physiological properties of fluid circulation in the crystalline lens in animals with regard to a phase of accommodation
The work was supported by Russian Fund of Fundamental Studies (Project No 16-06-00439). Background. Traditional ideas about the fluid circulation in the lens assume its movement through the lens capsul...
Substantiating the potential for a new technique (impedance oculopneumoplethysmography) to assist in diagnosing microvascular ciliary body ischemia
Purpose: To provide theoretical substantiation of the potential for a new technique, impedance oculopneumoplethysmography (OPPG), to assist in diagnosing microvascular ciliary body ischemia. Methods: Modeling the distri...